Department of Biochemistry and Department of Gastroenterology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Exp Mol Med. 2022 Oct;54(10):1601-1616. doi: 10.1038/s12276-022-00821-0. Epub 2022 Oct 21.
To date, more than 170 chemical modifications have been characterized in RNA, providing a new layer of gene expression regulation termed the 'epitranscriptome'. RNA modification detection methods and tools advance the functional studies of the epitranscriptome. According to the detection throughput and principles, existing RNA modification detection technologies can be categorized into four classes, including quantification methods, locus-specific detection methods, next-generation sequencing-based detection technologies and nanopore direct RNA sequencing-based technologies. In this review, we summarize the current knowledge about these RNA modification detection technologies and discuss the challenges for the existing detection tools, providing information for a comprehensive understanding of the epitranscriptome.
迄今为止，已有超过 170 种化学修饰被鉴定存在于 RNA 中，为基因表达调控提供了一个新的层面，称为“转录后修饰组”。RNA 修饰检测方法和工具促进了转录后修饰组的功能研究。根据检测通量和原理，现有的 RNA 修饰检测技术可分为四类，包括定量方法、基因座特异性检测方法、基于下一代测序的检测技术和基于纳米孔直接 RNA 测序的技术。在这篇综述中，我们总结了这些 RNA 修饰检测技术的最新知识，并讨论了现有检测工具面临的挑战，为全面了解转录后修饰组提供了信息。
