Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China.
State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Human Phenome Institute, Zhongshan Hospital, Fudan University, Shanghai, China.
J Dent Res. 2022 Dec;101(13):1580-1589. doi: 10.1177/00220345221110099. Epub 2022 Oct 20.
Disc displacement without reduction is a common disorder of the temporomandibular joint, causing clinical symptoms and sometimes condylar degeneration. In some cases, bone regeneration is detected following disc-repositioning procedures. Until now, however, systems-wide knowledge of the protein levels for condylar outcome with disc position is still lacking. Here, we performed comprehensive expression profiling of synovial fluid from 109 patients with disc displacement without reduction using high-resolution data-independent acquisition mass spectrometry and characterized differences in 1,714 proteins. Based on magnetic resonance imaging, samples were divided into groups with versus without condylar absorption and subgroups with versus without new bone. For the proteomic analysis, 32 proteins in groups presented with statistical significance (>2-fold, < 0.05). Pathways such as response to inorganic substances, blood coagulation, and estrogen signaling were significantly expressed in the group with bone absorption as compared with pathways such as regulation of body fluid levels, vesicle-mediated transport, and focal adhesion, which were enriched in the group without bone absorption. In subgroup analysis, 45 proteins of significant importance (>2-fold, < 0.05) were associated with pathways including would healing, glycolysis and gluconeogenesis, and amino acid metabolism. Combined with clinical examination, molecules such as acetyl-CoA carboxylase beta (ACACB) and transforming growth factor beta 1 (TGFB1) were related to features such as visual analog scale and maximum interincisal opening ( < 0.05). In addition, 7 proteins were examined by Western blotting, including progesterone immunomodulatory binding factor 1 (PIBF1), histidine-rich glycoprotein (HRG), and protein kinase C and casein kinase substrate in neurons 2 (PACSIN2). In conclusion, this study provides the first proteome analysis of condylar absorption at disc displacement without reduction and postoperative new bone formation after disc reposition. Integrated with clinical data, this analysis provides an important insight into the proteomics of condylar modification at disc position.
关节盘不可复位移是颞下颌关节的一种常见疾病，可导致临床症状，有时还会导致髁突退行性变。在某些情况下，关节盘复位术后可检测到骨再生。然而，迄今为止，对于关节盘位置与髁突结局的蛋白水平的系统级知识仍然缺乏。在这里，我们使用高分辨率数据非依赖性采集质谱法对 109 例不可复关节盘移位患者的滑液进行了全面的表达谱分析，并对 1714 种蛋白质的差异进行了特征描述。根据磁共振成像，将样本分为髁突吸收组和无吸收组，以及新骨形成组和无新骨形成组。在蛋白质组学分析中，有 32 种蛋白质在统计学上有显著差异（>2 倍，<0.05）。与无骨吸收组相比，骨吸收组中与无机物质反应、血液凝固和雌激素信号转导等途径显著表达，而无骨吸收组中则富含调节体液水平、囊泡介导运输和焦点粘附等途径。在亚组分析中，45 种具有重要意义的蛋白质（>2 倍，<0.05）与包括伤口愈合、糖酵解和糖异生以及氨基酸代谢在内的途径相关。结合临床检查，乙酰辅酶 A 羧化酶β（ACACB）和转化生长因子β 1（TGFB1）等分子与视觉模拟评分和最大开口度（<0.05）等特征相关。此外，还通过 Western 印迹法检测了 7 种蛋白质，包括孕激素免疫调节结合因子 1（PIBF1）、组氨酸丰富糖蛋白（HRG）、蛋白激酶 C 和神经元 2 中的酪蛋白激酶底物（PACSIN2）。总之，本研究首次对关节盘不可复位移且关节盘复位后出现新骨形成的髁突吸收进行了蛋白质组学分析。与临床数据相结合，该分析为关节盘位置下髁突改建的蛋白质组学提供了重要的见解。
