Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road 300, Nanjing, 210029, People's Republic of China.
Department of Gynecologic Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Sci Rep. 2022 Oct 22;12(1):17765. doi: 10.1038/s41598-022-21877-y.
CircRNAs are a group of endogenous small noncoding RNAs that are involved in multiple diseases including cancers. At present, the functions of circRNAs in breast cancer need to be further explored. In this study, 3 pairs of breast cancer and paracancer tissues with axillary lymph node metastasis were collected for circRNA high-throughput sequencing. We have identified 17,966 distinct circRNA candidates. Significant differential expressions were found in 136 circRNAs in breast cancer tissues relative to the matched paracancer tissues. We aslo identified differentially expressed 156 miRNAs and 1105 mRNAs in breast cancer tissues and normal breast tissues from public databases. Then we constructed a regulatory ceRNA network. 12 mRNAs were associated with prognosis of breast cancer. We also constructed a circRNAs-mediated subnetwork which might be related to prognosis of breast cancer. This article provides a better understanding of circRNAs-mediated ceRNA regulatory network by which circRNAs compete for endogenous RNAs in breast cancer.
环状 RNA 是一组内源性的小型非编码 RNA，参与多种疾病，包括癌症。目前，环状 RNA 在乳腺癌中的功能需要进一步探索。在这项研究中，收集了 3 对伴有腋窝淋巴结转移的乳腺癌和癌旁组织进行环状 RNA 高通量测序。我们已经鉴定出 17966 个不同的环状 RNA 候选物。与配对的癌旁组织相比，在乳腺癌组织中发现了 136 个环状 RNA 的显著差异表达。我们还从公共数据库中鉴定出了乳腺癌组织和正常乳腺组织中差异表达的 156 个 miRNA 和 1105 个 mRNA。然后我们构建了一个调控 ceRNA 网络。12 个 mRNAs 与乳腺癌的预后相关。我们还构建了一个环状 RNA 介导的子网络，可能与乳腺癌的预后有关。本文通过环状 RNA 竞争性结合内源性 RNA 来更好地理解环状 RNA 介导的 ceRNA 调控网络，从而阐明了环状 RNA 在乳腺癌中的作用机制。
