Omrizadeh Maryam, Mokhtari Pegah, Eftekhari-Yazdi Poopak, Chekini Zahra, Meybodi Anahita Mohseni
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Cell J. 2022 Sep 1;24(9):540-545. doi: 10.22074/cellj.2022.8077.
Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculogenesis. We aimed to the evaluation of the GDF9 and BMP15 expression in the granulosa cells (GCs) of DOR patients.
This case-control study included 14 women with DOR and 12 controls, who were between 28- 40 years of age undergoing controlled ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol. DOR patients were selected by the Bologna criteria. The GCs were extracted from the aspirated follicular fluids and RNA isolated from this. The fold change of gene expressions was assessed by real-time polymerase chain reaction (PCR).
GDF9 expression in patients was 0.23 times lower than the control group, which was significant (P<0.0001). BMP15 expression in patients was 0.32 times lower than the control group, which was significant (P<0.0001). The number of archived oocytes, MII, and two pronuclei (PN) embryos was higher in the control group and these differences were statistically significant (P<0.05).
Given that GDF9 and BMP15 are specifically involved during follicular recruitmen., we expect expression of these two genes in DOR patients which is greatly reduced by reducing follicular reserve.
卵巢储备功能减退（DOR）是辅助生殖技术中遇到的一个具有挑战性的问题。生长分化因子9（GDF9）和骨形态发生蛋白15（BMP15）属于转化生长因子-β（TGF-β）超家族，对卵泡发生至关重要。我们旨在评估DOR患者颗粒细胞（GCs）中GDF9和BMP15的表达。
本病例对照研究纳入了14例DOR患者和12例对照，年龄在28至40岁之间，采用促性腺激素释放激素（GnRH）拮抗剂方案进行控制性卵巢刺激。DOR患者根据博洛尼亚标准选择。从抽吸的卵泡液中提取GCs，并从中分离RNA。通过实时聚合酶链反应（PCR）评估基因表达的倍数变化。
患者中GDF9的表达比对照组低0.23倍，差异有统计学意义（P<0.0001）。患者中BMP15的表达比对照组低0.32倍，差异有统计学意义（P<0.0001）。对照组中存档卵母细胞、MII期和双原核（PN）胚胎的数量较多，这些差异具有统计学意义（P<0.05）。
鉴于GDF9和BMP15在卵泡募集过程中发挥特定作用，我们预期这两个基因在DOR患者中的表达会因卵泡储备减少而大幅降低。
