年轻低预后患者的卵泡液和颗粒细胞中 GDF9 和 BMP15 降低，以及 IVF-ET 的结局。
Decreased GDF9 and BMP15 in follicle fluid and granulosa cells and outcomes of IVF-ET among young patients with low prognosis.
机构信息
Reproductive Medicine Center, Sichuan Provincial Women's and Children's Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, 610045, Sichuan, China.
Women and Children's Health Management Department, Sichuan Provincial Women's and Children's Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, 610045, Sichuan, China.
出版信息
J Assist Reprod Genet. 2023 Mar;40(3):567-576. doi: 10.1007/s10815-023-02723-0. Epub 2023 Jan 23.
PURPOSE
To analyze the level of growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) in follicle fluid (FF) and granulosa cells (GCs) derived from young patients with low prognosis for in vitro fertilization and embryo transfer (IVF-ET) treatment.
METHODS
A prospective cohort study was carried out by enrolling 52 young patients with low prognosis according to the POSEIDON classification group 3 (low prognosis group) and 51 young patients with normal ovarian reserve (control group). The concentration of the GDF9 and BMP15 proteins in FF was determined by enzyme-linked immunosorbent assay. The mRNA level of the GDF9 and BMP15 in the GCs was measured by quantitative real-time PCR.
RESULTS
The concentration of GDF9 (1026.72 ± 159.12 pg/mL vs. 1298.06 ± 185.41 pg/mL) and BMP15 (685.23 ± 143.91 pg/mL vs. 794.37 ± 81.79 pg/mL) in FF and the mRNA level of GDF9 and BMP15 in the GCs and the live birth rate per treatment cycle started (30.77% vs. 50.98%) and oocytes retrieved (4.25 ± 1.91 vs.12.04 ± 4.24) were significantly lower, whereas the canceled cycle rate was significantly higher (9.62% vs. 0) in the low prognosis group compared with the control group (P < 0.05). The expression of GDF9 and BMP15 in the ovary was positively correlated with live birth (P < 0.05).
CONCLUSION
The expression of GDF9 and BMP15 in the ovary was decreased in young patients with low prognosis accompanied by a poorer outcome of IVF-ET treatment.
TRIAL REGISTRATION
ChiCTR1800016107 (Chinese Clinical Trial Registry), May 11, 2018. ( http://www.chictr.org.cn/edit.aspx?pid=27216&htm=4 ).
目的
分析体外受精-胚胎移植（IVF-ET）治疗预后不良的年轻患者的卵泡液（FF）和颗粒细胞（GC）中生长分化因子 9（GDF9）和骨形态发生蛋白 15（BMP15）的水平。
方法
采用前瞻性队列研究，根据 POSEIDON 分类组 3（预后不良组）纳入 52 例预后不良的年轻患者，根据正常卵巢储备纳入 51 例年轻患者（对照组）。通过酶联免疫吸附试验测定 FF 中 GDF9 和 BMP15 蛋白的浓度。通过实时定量 PCR 测定 GC 中 GDF9 和 BMP15 的 mRNA 水平。
结果
FF 中 GDF9（1026.72±159.12 pg/mL 比 1298.06±185.41 pg/mL）和 BMP15（685.23±143.91 pg/mL 比 794.37±81.79 pg/mL）浓度以及 GDF9 和 BMP15 在 GC 中的 mRNA 水平、每个治疗周期开始的活产率（30.77%比 50.98%）和取卵数（4.25±1.91 比 12.04±4.24）均明显降低，而取消周期率（9.62%比 0）明显升高（P<0.05）。卵巢中 GDF9 和 BMP15 的表达与活产呈正相关（P<0.05）。
结论
伴有 IVF-ET 治疗结局较差的年轻患者卵巢中 GDF9 和 BMP15 的表达降低。
试验注册
ChiCTR1800016107（中国临床试验注册中心），2018 年 5 月 11 日（http://www.chictr.org.cn/edit.aspx?pid=27216&htm=4）。
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