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评估一种尼帕病毒可溶性G疫苗候选物在小鼠和猪体内的免疫原性及保护作用。

Assessment of the immunogenicity and protection of a Nipah virus soluble G vaccine candidate in mice and pigs.

作者信息

Gao Zihan, Li Tao, Han Jicheng, Feng Sheng, Li Letian, Jiang Yuhang, Xu Zhiqiang, Hao Pengfei, Chen Jing, Hao Jiayi, Xu Peng, Tian Mingyao, Jin Ningyi, Huang Weijin, Li Chang

机构信息

Research Unit of Key Technologies for Prevention and Control of Virus Zoonoses, Chinese Academy of Medical Sciences, Changchun Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, Changchun, China.

Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

出版信息

Front Microbiol. 2022 Oct 6;13:1031523. doi: 10.3389/fmicb.2022.1031523. eCollection 2022.

DOI:10.3389/fmicb.2022.1031523
PMID:36274696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9583134/
Abstract

Nipah virus (NiV) is a newly emerged extremely dangerous zoonotic pathogen highly fatal to humans. Currently, no approved vaccine is available against NiV. This study employed a mammalian eukaryotic system to express NiV soluble G glycoprotein (NiV-sG), using CpG oligodeoxynucleotides (CpG)/Aluminum salt (Alum) as adjuvants to obtain a recombinant subunit vaccine candidate. We also evaluated the immunogenicity and efficacy of the protein in mice and pigs. The results showed that humoral and cellular immune responses were induced in all the vaccination groups in two animal models. The levels of specific and neutralizing antibodies and the proliferation levels of T helper(Th) cells were significantly higher than those in the control group. The protective efficacy of the subunit vaccines evaluated in the pseudovirus infection mouse model strongly suggested that this vaccine could provide protective immunity against NiV. A neoadjuvant (HTa) based on liposomes and cholera toxin combined with CpG/Alum was exploited and evaluated in mice. The neoadjuvant group showed a more protective efficacy than the CpG/Alum group. The aforementioned results indicated that the subunit vaccine could be used as a promising candidate vaccine for preventing Nipah virus infection.

摘要

尼帕病毒（NiV）是一种新出现的对人类极具致命性的人畜共患病原体。目前，尚无获批的针对尼帕病毒的疫苗。本研究采用哺乳动物真核系统表达尼帕病毒可溶性G糖蛋白（NiV-sG），使用CpG寡脱氧核苷酸（CpG）/铝盐（明矾）作为佐剂来获得一种重组亚单位候选疫苗。我们还评估了该蛋白在小鼠和猪体内的免疫原性和效力。结果表明，在两种动物模型的所有疫苗接种组中均诱导了体液免疫和细胞免疫反应。特异性抗体和中和抗体水平以及辅助性T细胞（Th）的增殖水平均显著高于对照组。在伪病毒感染小鼠模型中评估的亚单位疫苗的保护效力有力地表明，该疫苗可提供针对尼帕病毒的保护性免疫。开发了一种基于脂质体和霍乱毒素并结合CpG/明矾的新型佐剂（HTa）并在小鼠中进行了评估。新型佐剂组显示出比CpG/明矾组更强的保护效力。上述结果表明，该亚单位疫苗可作为预防尼帕病毒感染的一种有前景的候选疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/ead421980f0e/fmicb-13-1031523-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/ee41dafe3831/fmicb-13-1031523-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/627d8bc0f039/fmicb-13-1031523-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/f8ed34e151ad/fmicb-13-1031523-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/99e64e2ebe1b/fmicb-13-1031523-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/ead421980f0e/fmicb-13-1031523-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/ee41dafe3831/fmicb-13-1031523-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/627d8bc0f039/fmicb-13-1031523-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/f8ed34e151ad/fmicb-13-1031523-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/99e64e2ebe1b/fmicb-13-1031523-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b08/9583134/ead421980f0e/fmicb-13-1031523-g005.jpg

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