Tang Weimin, Wei Yanjun, Lu Wenjing, Chen Di, Ye Qin, Zhang Cen, Chen Yufeng, Xiao Chaogeng
National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang 310052, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
Food Chem X. 2022 Oct 13;16:100477. doi: 10.1016/j.fochx.2022.100477. eCollection 2022 Dec 30.
Naringin is potential functional and therapeutic ingredient, has low bioavailability because of poor aqueous solubility. In this study, an ovalbumin (OVA)-carboxymethyl konjac glucomannan (CKGM) nano-delivery system was developed to enhance the bioavailability of naringin. The effects of proportion (OVA: CKGM), pH and naringin concentration were studied on the formation, encapsulation efficiency (EE) and bioaccessibility of OVA/CKGM-Naringin nanoparticles (OVA/CKGM-Naringin NPs). Its morphology and size were viewed by Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM). The cross-linkage between OVA and CKGM was verified by Fourier Transform Infrared Spectroscopy (FTIR) and Fluorescence Intensity analysis. The size of OVA/CKGM-Naringin NPs were 463.83 ± 18.50 nm (Polydispersity Index-PDI, 0.42 ± 0.05). It indicated that 2:1 of OVA: CKGM, pH 3 and 7 mg/mL of naringin concentration were optimized processing parameters of OVA/CKGM-Naringin NPs with EE (97.90 ± 2.97 %) and remarkably improved bioaccessibility (85.01 ± 2.52 %). The OVA/CKGM-Naringin NPs was energy efficiently prepared and verified as an ideal carrier of naringin.
柚皮苷是一种潜在的功能性和治疗性成分,由于其水溶性差,生物利用度较低。在本研究中,开发了一种卵清蛋白(OVA)-羧甲基魔芋葡甘露聚糖(CKGM)纳米递送系统,以提高柚皮苷的生物利用度。研究了比例(OVA:CKGM)、pH值和柚皮苷浓度对OVA/CKGM-柚皮苷纳米颗粒(OVA/CKGM-Naringin NPs)的形成、包封率(EE)和生物可及性的影响。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察其形态和大小。通过傅里叶变换红外光谱(FTIR)和荧光强度分析验证了OVA和CKGM之间的交联。OVA/CKGM-柚皮苷纳米粒的粒径为463.83±18.50nm(多分散指数-PDI,0.42±0.05)。结果表明,OVA:CKGM为2:1、pH值为3、柚皮苷浓度为7mg/mL是OVA/CKGM-柚皮苷纳米粒的优化工艺参数,其包封率为(97.90±2.97%),生物可及性显著提高(85.01±2.52%)。OVA/CKGM-柚皮苷纳米粒制备高效节能,被验证为柚皮苷的理想载体。