影响脂肪来源基质血管部分间充质干细胞数量的因素。
Factors Affecting the Population of Mesenchymal Stem Cells in Adipose-Derived Stromal Vascular Fraction.
机构信息
Department of Plastic, Reconstructive and Aesthetic Surgery, Acıbadem University Faculty of Medicine, Acıbadem Altunizade Hospital, İstanbul, Turkey
Department of Hematology, Acıbadem University Faculty of Medicine, Acıbadem Labcell Cellular Therapy Facility, İstanbul, Turkey
出版信息
Balkan Med J. 2022 Nov 7;39(6):386-392. doi: 10.4274/balkanmedj.galenos.2022.2022-5-50. Epub 2022 Oct 24.
BACKGROUND
Recently, the adipose tissue-derived stromal vascular fraction has become the most popular source for obtaining mesenchymal stem cells because it is less expensive and is easier to perform than bone marrow concentrate harvesting. However, no study has investigated the factors affecting the mesenchymal stem cell population in adipose tissue derived stromal vascular fraction. Understanding the interaction of patient factors with the mesenchymal stem cell count and cell viability in adipose tissue-derived stromal vascular fraction could provide crucial information for surgeons to improve patient selection and outcomes.
AIMS
To evaluate the factors affecting the mesenchymal stem cell count, total cell count, and cell viability in adipose tissue-derived stromal vascular fraction.
STUDY DESIGN
Retrospective cross-sectional study.
METHODS
This study retrospectively reviewed the medical records of 30 patients who underwent liposuction to harvest adipose tissue-derived stromal vascular fraction at our stem cell center. Operative variables, such as lipoaspirate amount and donor areas from the stromal vascular fraction harvesting site, included the entire abdomen and lower abdomen. We recorded the mesenchymal stem cell population, cell viability, and cell count of stromal vascular fraction, and we analyzed the results to determine statistical significance.
RESULTS
The factors that were found to be significantly related are as follows; between cell number and age (=0.001) and amount of lipoaspirate (<0.001); between cell viability and body mass index (=0.005) and hypertension (=0.047); and between coronary artery disease and mesenchymal stem cell counts ( = 0.028).
CONCLUSION
The relationship of patient factors (age, body mass index, hypertension, and coronary artery disease) with cell viability and mesenchymal stem cell counts may be important for clinical applications. However, the effect of medications on these relationships should be investigated in larger studies.
背景
最近，脂肪组织来源的基质血管部分已成为获取间充质干细胞的最受欢迎来源，因为它比骨髓浓缩物采集更便宜、更容易进行。然而，尚无研究探讨影响脂肪组织来源的基质血管部分中间充质干细胞群体的因素。了解患者因素与脂肪组织来源的基质血管部分中的间充质干细胞计数和细胞活力的相互作用，可以为外科医生提供重要信息，以改善患者选择和结果。
目的
评估影响脂肪组织来源的基质血管部分中间充质干细胞计数、总细胞计数和细胞活力的因素。
研究设计
回顾性横截面研究。
方法
本研究回顾性分析了在我们的干细胞中心接受脂肪抽吸术以收获脂肪组织来源的基质血管部分的 30 名患者的病历。手术变量，如脂肪抽吸量和基质血管部分采集部位的供区，包括整个腹部和下腹部。我们记录了基质血管部分的间充质干细胞群体、细胞活力和细胞计数，并对结果进行了分析，以确定统计学意义。
结果
发现与细胞数量显著相关的因素如下：细胞数量与年龄（=0.001）和脂肪抽吸量（<0.001）之间；细胞活力与体重指数（=0.005）和高血压（=0.047）之间；以及冠心病与间充质干细胞计数（=0.028）之间。
结论
患者因素（年龄、体重指数、高血压和冠心病）与细胞活力和间充质干细胞计数的关系可能对临床应用很重要。然而，应在更大的研究中调查药物对这些关系的影响。
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