Sproat B S, Beijer B, Rider P
Nucleic Acids Res. 1987 Aug 11;15(15):6181-96. doi: 10.1093/nar/15.15.6181.
Synthetic routes to the four appropriately protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) have been developed. The structures of all intermediates were confirmed by 13C n.m.r. spectroscopy. These building blocks have been used to prepare 5'-amino-oligodeoxyribonucleotides, which can be coupled to a wide variety of compounds, in particular metal cluster derivatives, but also fluorophores and biotin derivatives, thus generating a variety of very useful probes. Brief mention is made of a tetrairidium cluster derivative of 5'-amino-d[CCGATATCGG], which has been cocrystallised with EcoRV, and will be used for electron microscopy studies.
已开发出合成四种适当保护的5'-氨基-2',5'-二脱氧核糖核苷-3'-O-(2-氰基乙基N,N-二异丙基磷酰胺)的路线。所有中间体的结构均通过13C核磁共振光谱得到证实。这些构建模块已用于制备5'-氨基-寡脱氧核糖核苷酸,其可与多种化合物偶联,特别是金属簇衍生物,也包括荧光团和生物素衍生物,从而产生各种非常有用的探针。简要提及了5'-氨基-d[CCGATATCGG]的一种四铱簇衍生物,它已与EcoRV共结晶,并将用于电子显微镜研究。