将非放射性标记物连接到合成寡脱氧核糖核苷酸5'末端的高效方法。
Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.
作者信息
Agrawal S, Christodoulou C, Gait M J
出版信息
Nucleic Acids Res. 1986 Aug 11;14(15):6227-45. doi: 10.1093/nar/14.15.6227.
Abstract
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.
摘要
描述了两种连接分子的合成方法，这两种连接分子可用于将生物素和荧光团等非放射性标记物特异性连接到合成寡脱氧核糖核苷酸的5'末端。这些连接分子的设计使得它们能够在使用商用DNA合成仪进行固相合成的最后一步与寡核苷酸偶联。使用抗生物素杂交瘤/过氧化物酶检测系统可以提高生物素检测的灵敏度。
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PARTIAL PURIFICATION OF SOLUBLE RNA.可溶性核糖核酸的部分纯化
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