Production Engineering and Plant Genetics Department, Faculty of Agriculture and Natural Resources, Lorestan University, P.O. Box 465, Khorramabad, Iran.
Department of Plant Production & Genetics, Faculty of Agriculture, Agricultural Sciences & Natural Resources, University of Khuzestan, Mollasani, Iran.
Sci Rep. 2022 Oct 24;12(1):17803. doi: 10.1038/s41598-022-20314-4.
The anti-cancer vinblastine and vincristine alkaloids can only be naturally found in periwinkle (Catharanthus roseus). Both of these alkaloids' accumulations are known to be influenced by salicylic acid (SA). The transcriptome data to reveal the induction effect (s) of SA, however, seem restricted at this time. In this study, the de novo approach of transcriptome assembly was performed on the RNA-Sequencing (RNA-Seq) data in C. roseus. The outcome demonstrated that SA treatment boosted the expression of all the genes in the Terpenoid Indole Alkaloids (TIAs) pathway that produces the vinblastine and vincristine alkaloids. These outcomes supported the time-course measurements of vincristine alkaloid, the end product of the TIAs pathway, and demonstrated that SA spray had a positive impact on transcription and alkaloid synthesis. Additionally, the abundance of transcription factor families including bHLH, C3H, C2H2, MYB, MYB-related, AP2/ ERF, NAC, bZIP, and WRKY suggests a role for a variety of transcription families in response to the SA stimuli. Di-nucleotide and tri-nucleotide SSRs were the most prevalent SSR markers in microsatellite analyses, making up 39% and 34% of all SSR markers, respectively, out of the 77,192 total SSRs discovered.
抗癌长春碱和长春新碱生物碱只能在长春花（长春花）中自然发现。这两种生物碱的积累都被认为受水杨酸（SA）的影响。然而，目前似乎仅限于揭示 SA 诱导效应（s）的转录组数据。在这项研究中，对长春花的 RNA 测序（RNA-Seq）数据进行了从头组装转录组的方法。结果表明，SA 处理促进了产生长春碱和长春新碱生物碱的萜类吲哚生物碱（TIAs）途径中所有基因的表达。这些结果支持了 TIAs 途径的终产物长春新碱生物碱的时程测量，并表明 SA 喷雾对转录和生物碱合成有积极影响。此外，包括 bHLH、C3H、C2H2、MYB、MYB 相关、AP2/ERF、NAC、bZIP 和 WRKY 在内的转录因子家族的丰度表明，各种转录因子家族在响应 SA 刺激方面发挥作用。二核苷酸和三核苷酸 SSR 是微卫星分析中最常见的 SSR 标记，在发现的 77192 个总 SSR 中，分别占 SSR 标记的 39%和 34%。
