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从三级护理医院实验室送检的培养样本中分离真菌。

Fungal isolation from wound samples submitted for culture at a tertiary care hospital laboratory.

机构信息

Department of Pathology and Laboratory Medicine The Aga Khan University Hospital, Karachi, Pakistan.

Department of Pathology and Laboratory Medicine, Aga Khan University, Karachi, Pakistan.

出版信息

J Pak Med Assoc. 2022 Aug;72(8):1622-1625. doi: 10.47391/JPMA.2105.

Abstract

Fungal wound infections are increasing worldwide. The aim of this retrospective study, conducted at the Aga Khan University laboratory, Karachi, Pakistan, was to determine the frequency of fungal isolation in wound specimens. Data of wound samples received for culture from all over the country between September and October 2018 was reviewed. Samples were processed for bacterial cultures and additionally inoculated on Sabouraud's dextrose medium. Demographic information, medical history and information on the type of wound was collected. A total of 140 cases were included, of which 87 (81%) were culture positive, while 10 (7%) cases yielded fungi. Burn and blast wounds had the highest proportion of fungal isolation, i.e. 2 out of 4 (50%). Candida species were the most common fungi (n=4), followed by Fusarium species (n=3). This study reports an alarming rate of fungal wound infections. As fungal necrotising wound infections have high morbidity and mortality, it is, therefore, important to accurately diagnose and treat such infections in local setting.

摘要

真菌性伤口感染在全球范围内呈上升趋势。本研究在巴基斯坦卡拉奇的 Aga Khan 大学实验室进行,旨在确定伤口标本中真菌分离的频率。回顾了 2018 年 9 月至 10 月间来自全国各地的用于培养的伤口样本的数据。对样本进行了细菌培养,并额外接种在沙氏葡萄糖琼脂培养基上。收集了人口统计学信息、病史以及伤口类型的信息。共纳入 140 例,其中 87 例(81%)培养阳性,10 例(7%)检出真菌。烧伤和爆炸伤的真菌分离率最高,即 4 例中的 2 例(50%)。最常见的真菌为念珠菌属(n=4),其次为镰刀菌属(n=3)。本研究报告了真菌性伤口感染令人震惊的发生率。由于真菌性坏死性伤口感染的发病率和死亡率较高,因此在当地环境中准确诊断和治疗此类感染非常重要。

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