γ-氨基丁酸处理通过调节抗坏血酸代谢诱导采后猕猴桃（中华猕猴桃 cv. 红阳）的耐冷性。

γ-Aminobutyric acid treatment induced chilling tolerance in postharvest kiwifruit (Actinidia chinensis cv. Hongyang) via regulating ascorbic acid metabolism.

机构信息

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China.

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

出版信息

Food Chem. 2023 Mar 15;404(Pt B):134661. doi: 10.1016/j.foodchem.2022.134661. Epub 2022 Oct 19.

DOI:10.1016/j.foodchem.2022.134661

PMID:36283321

Abstract

The effect of γ-Aminobutyric acid (GABA) treatment on ascorbic acid (AsA) metabolism and chilling injury in postharvest kiwifruit was studied. The results revealed that kiwifruit treated with GABA displayed higher chilling tolerance and better quality maintenance as compared to the controls. Higher AsA was observed in GABA-treated fruit which was beneficial to cell membrane protection and damage alleviation against chilling mediated oxidative stress. Gene expression analysis found the increased expression of AsA anabolic and regenerative genes and down-regulation of its catabolic genes together could contribute to the elevation of AsA levels in kiwifruit after GABA treatment. In addition, the transcripts of several candidate transcription factors such as bHLHs and HZ1 involved in AsA biosynthesis were also enhanced by GABA treatment. Collectively, our results indicated that GABA induced chilling tolerance in postharvest kiwifruit due to the higher AsA content by positively regulating ascorbate metabolic genes and candidate transcription factors.

摘要

研究了γ-氨基丁酸（GABA）处理对采后猕猴桃抗坏血酸（AsA）代谢和冷害的影响。结果表明，与对照相比，用 GABA 处理的猕猴桃具有更高的耐冷性和更好的品质保持。在 GABA 处理的果实中观察到更高的抗坏血酸（AsA）水平，这有利于细胞膜保护，并减轻冷介导的氧化应激对细胞的损伤。基因表达分析发现，AsA 合成和再生基因的表达增加，而其分解代谢基因的表达下调，这可能有助于 GABA 处理后猕猴桃中 AsA 水平的升高。此外，参与 AsA 生物合成的几个候选转录因子（如 bHLHs 和 HZ1）的转录本也被 GABA 处理增强。总之，我们的结果表明，由于 GABA 通过正向调节抗坏血酸代谢基因和候选转录因子，使采后猕猴桃中的 AsA 含量增加，从而诱导其对冷害的耐受性。