Aghdam Morteza Soleimani, Asle-Mohammadi Zeinab, Ebrahimi Amin, Razavi Farhang
Department of Horticultural Science, Imam Khomeini International University, Qazvin, 34148- 96818, Iran.
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Sci Rep. 2025 Jan 23;15(1):2894. doi: 10.1038/s41598-025-87542-2.
This study investigated the mechanisms employed by exogenous dopamine application in alleviating chilling injury in kiwifruits during storage at 1 °C for 120 days. Our results indicated that dopamine treatment at 150 µM alleviated chilling injury in kiwifruits during storage at 1 °C for 120 days. By 150 µM dopamine application, higher SUMO E3 ligase (SIZ1) and target of rapamycin (TOR) genes expression accompanied by lower poly(ADP-Ribose) polymerase 1 (PARP1) and sucrose non-fermenting 1-related kinase 1 (SnRK1) genes expression was associated with higher salicylic acid, ATP, NADPH and proline accumulation in kiwifruits during storage at 1 °C for 120 days. In addition, higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity arising from higher phenols and flavonoids accumulation in kiwifruits treated with 150 µM dopamine could be ascribed to higher phenylalanine ammonia-lyase (PAL) enzyme activity. Additionally, lower endogenous hydrogen peroxide (HO) accumulation along with higher ascorbic acid accumulation in kiwifruits treated with 150 µM dopamine could be attributed to lower superoxide dismutase (SOD) along with higher catalase (CAT) enzymes activity. Moreover, lower phospholipase D (PLD) and lipoxygenase (LOX) genes expression in kiwifruits treated with 150 µM dopamine was accompanied with membrane integrity preservation as evidenced by lower electrolyte leakage and malondialdehyde (MDA) accumulation. Therefore, exogenous dopamine could be employed as a potential technique for alleviating chilling injury in kiwifruits during cold storage.
本研究探究了外源施加多巴胺在减轻猕猴桃于1℃贮藏120天期间冷害方面所采用的机制。我们的结果表明，150μM多巴胺处理减轻了猕猴桃在1℃贮藏120天期间的冷害。通过施加150μM多巴胺，较高的小泛素样修饰蛋白E3连接酶（SIZ1）和雷帕霉素靶蛋白（TOR）基因表达，伴随着较低的聚（ADP - 核糖）聚合酶1（PARP1）和蔗糖非发酵1相关激酶1（SnRK1）基因表达，与猕猴桃在1℃贮藏120天期间较高的水杨酸、ATP、NADPH和脯氨酸积累有关。此外，150μM多巴胺处理的猕猴桃中，由于酚类和黄酮类物质积累增加而产生的较高的2,2 - 二苯基 - 1 - 苦基肼（DPPH）清除能力，可归因于较高的苯丙氨酸解氨酶（PAL）酶活性。另外，150μM多巴胺处理的猕猴桃中较低的内源性过氧化氢（H₂O₂）积累以及较高的抗坏血酸积累，可归因于较低的超氧化物歧化酶（SOD）以及较高的过氧化氢酶（CAT）活性。此外，150μM多巴胺处理的猕猴桃中较低的磷脂酶D（PLD）和脂氧合酶（LOX）基因表达，伴随着膜完整性的保持，这由较低的电解质渗漏和丙二醛（MDA）积累所证明。因此，外源多巴胺可作为一种潜在技术用于减轻猕猴桃冷藏期间的冷害。
