A novel 3D-electrospun nanofibers-scaffold grafted with Royal Jelly: improve hydrophilicity of the nanofibers-scaffold and proliferation of HUVEC cell line.

Tissue engineering is one of the most important medical rehabilitation tools that includes two vital components of scaffolding and cell growth stimulants. Therefore, designing a more intelligent, portable, monitorable, and safe scaffold that can release growth factors is a key step in achieving an acceptable level of cells for treating patients. In this study, a nanofibers-grafted scaffold was prepared with two-nozzle electrospinning to serve as a tissue engineering scaffold. Fundamental physical characterizations were carried out by scanning electron microscopy (SEM), pore diameter determination, and FT-IR. Fundamental physical characterization revealed that the nanofibers-scaffolds grafted with Royal Jelly significantly increased hydrophilicity, but the porosity of the novel-nanofibers did not alter significantly than the nanofibers without Royal Jelly. Based on the MTT assay results, cell growth, survival, and proliferation of the HUVEC Cell line were increased in the nanofibers scaffold grafted with Royal Jelly. Together, these findings highlight the potential of our novel scaffold for tissue engineering applications.