Parasher Nitin, Kaushik Priya, Singh Naveen Kumar, Yadav Lalit, Bhurer Yadav Bibek, Suri Arpita
Department of Biochemistry, Faculty of Medicine & Health Sciences, SGT University, Gurugram, Haryana, India.
Department of Pathology, Faculty of Medicine & Health Sciences, SGT University, Gurugram, Haryana, India.
Horm Mol Biol Clin Investig. 2022 Oct 27;44(1):39-43. doi: 10.1515/hmbci-2022-0012. eCollection 2023 Mar 1.
Preeclampsia is a multisystem illness that manifests in the third trimester of pregnancy after 20 weeks of gestation and is marked by proteinuria and hypertension (PE). Changes in lifestyle, such as eating a high-calorie diet and delaying delivery, have raised the likelihood of developing PE. Eclampsia, abrupt renal failure, thromboembolic episodes leading to cardiac and brain problems, pulmonary embolism, and coagulopathy associated with HELLP syndrome are a few of the complications that might follow preeclampsia in pregnant moms. The objects of this study is to estimate and correlate the levels of NGAL (neutrophil gelatinase associated lipocalin), IMA (ischemia modified albumin) and Uric acid in prreclampsia.
40 diagnosed cases of preeclampsia and 40 healthy age and gestational age matched healthy controls were included in the study. Blood samples were collected from them and serum NGAL, IMA and Uric acid levels were estimated. Estimation of NGAL (neutrophil gelatinase associated lipocalin), IMA (ischemia modified albumin) was done by commercially available ELISA kits standard spectrophotometry methods in autoanalyzer Mind ray BS300 using commercially available kits.
The parameters of NGAL and IMA were significantly increased in patients with PE (p<0.001) when compared with the healthy control subjects. γ-glutamyl transferases and OPN were found in patients with ALD (p<0.001) when compared with the control subjects. OPN showed significant positive correlations with AST (r=0.76, p<0.001), ALT (r=0.64 p<0.001), ALP (r=0.68, p<0.001), and GGT (r=0.61, p<0.001).
The current study focuses on the roles of NGAL and IMA, two sensitive markers of kidney injury that are particularly useful in identifying widespread endothelial dysfunction. As a result, the pattern of elevated NGAL and IMA levels can be useful for diagnosis.
子痫前期是一种多系统疾病，在妊娠20周后的妊娠晚期出现，其特征为蛋白尿和高血压（PE）。生活方式的改变，如高热量饮食和延迟分娩，增加了患子痫前期的可能性。子痫、急性肾衰竭、导致心脏和脑部问题的血栓栓塞事件、肺栓塞以及与HELLP综合征相关的凝血病是妊娠母亲子痫前期可能引发的一些并发症。本研究的目的是评估子痫前期患者中性粒细胞明胶酶相关脂质运载蛋白（NGAL）、缺血修饰白蛋白（IMA）和尿酸水平，并进行相关性分析。
本研究纳入40例确诊的子痫前期病例以及40例年龄和孕周匹配的健康对照。采集他们的血样并检测血清NGAL、IMA和尿酸水平。使用迈瑞BS300全自动分析仪，通过商业可用的ELISA试剂盒和标准分光光度法检测NGAL（中性粒细胞明胶酶相关脂质运载蛋白）和IMA（缺血修饰白蛋白）。
与健康对照相比，子痫前期患者的NGAL和IMA参数显著升高（p<0.001）。与对照相比，酒精性肝病（ALD）患者中发现γ-谷氨酰转移酶和骨桥蛋白（OPN）升高（p<0.001）。OPN与天冬氨酸转氨酶（AST，r=0.76，p<0.001）、丙氨酸转氨酶（ALT，r=0.64，p<0.001）、碱性磷酸酶（ALP，r=0.68，p<0.001）和γ-谷氨酰转移酶（GGT，r=0.61，p<0.001）呈显著正相关。
本研究聚焦于NGAL和IMA这两种肾损伤敏感标志物的作用，它们在识别广泛的内皮功能障碍方面尤为有用。因此，NGAL和IMA水平升高的模式可用于诊断。
