Yan Cheng, Yang Yan, Tang Yunxiang, Zheng Xiaojing, Xu Bin
Department of Oncology and Hematology, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing 400014, China.
Department of Nuclear Medicine, Chongqing Emergency Medical Center, Chongqing University Central Hospital, Chongqing 400014, China.
J Oncol. 2022 Oct 15;2022:2909095. doi: 10.1155/2022/2909095. eCollection 2022.
Common chromophobe renal cell carcinoma (chRCC) has a good prognosis when cured by surgery. However, clinical practice shows that a small number of patients with chRCC will produce metastasis, and the prognosis after metastasis is poor. In this regard, we try to find potential biological targets to prevent CRCC metastasis. In this experiment, we analyzed the clinical traits and gene expression data of chRCC samples which were provided by the TCGA database by the WGCNA method. On this basis, we selected MEtan, a module with a significant positive correlation with the M phase of chRCC, for subsequent analysis. The MEtan module genes in the biological process of chRCC were mainly related to steroid metabolic process, cholesterol metabolic process and STEM cell differentiation. KEGG analysis showed that these genes were mainly enriched in cancer-related signaling pathways, such as Neuroactive Ligand-receptor interaction, cAMP signaling pathway, and Wnt signaling pathway. Subsequently, we mapped the PPI interaction network and screened the key gene beta-arrestin 2 (ARRB2). Expression analysis showed that there was a significantly increased expression of ARRB2 in chRCC patients in comparison to the normal group. Expression survival analysis indicated that ARRB2 was inversely associated with overall survival. We firmly believe that the key genes identified in this study would be able to provide new clues and research basis for the treatment of chRCC.
常见的嫌色性肾细胞癌（chRCC）通过手术治愈后预后良好。然而，临床实践表明，少数chRCC患者会发生转移，转移后的预后较差。对此，我们试图寻找潜在的生物学靶点以预防chRCC转移。在本实验中，我们通过WGCNA方法分析了TCGA数据库提供的chRCC样本的临床特征和基因表达数据。在此基础上，我们选择了与chRCC的M期显著正相关的模块MEtan进行后续分析。chRCC生物学过程中的MEtan模块基因主要与类固醇代谢过程、胆固醇代谢过程和干细胞分化有关。KEGG分析表明，这些基因主要富集于癌症相关信号通路，如神经活性配体-受体相互作用、cAMP信号通路和Wnt信号通路。随后，我们绘制了PPI相互作用网络并筛选出关键基因β-抑制蛋白2（ARRB2）。表达分析表明，与正常组相比，chRCC患者中ARRB2的表达显著增加。表达生存分析表明，ARRB2与总生存期呈负相关。我们坚信，本研究中鉴定出的关键基因能够为chRCC的治疗提供新的线索和研究依据。
