Wu Jinyan, Cai Bangrong, Zhang Ang, Zhao Peng, Du Yan, Liu Xuefang, Zhao Di, Yang Liu, Liu Xinguang, Li Jiansheng
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of China, Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, China.
Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou, China.
Int J Anal Chem. 2022 Oct 15;2022:1423801. doi: 10.1155/2022/1423801. eCollection 2022.
Chronic obstructive pulmonary disease (COPD) has high morbidity and mortality and presents a threat to human health worldwide. Numerous clinical trials have confirmed that Bufei Yishen formula (BYF), an herbal medicine, can alleviate the symptoms of COPD by reducing oxidative stress-mediated inflammation. However, the active components of BYF remain unclear. We developed an efficient ultrahigh-performance liquid chromatography Q-Extractive Orbitrap mass spectrometry method to identify the composition of BYF and determine its antioxidant profile through an offline screening strategy based on 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH)-liquid chromatography-mass spectrometry. In total, 189 compounds were identified in BYF extract, including 83 flavonoids, 24 lignans, 20 alkaloids, 15 saponins, 11 terpenoid, 10 saccharides, eight lipids, seven organic acids, two coumarins, two amino acids, and seven other compounds. Among them, 79 compounds were found to have a potential antioxidant activity. validation indicated that the free radical scavenging activities of rosmarinic acid and calycosin were similar to that of the positive control (DPPH IC = 25.72 ± 1.02 and 147.23 ± 25.12 g/mL, respectively). Furthermore, calycosin had a high content in serum after the oral administration of BYF, indicating that calycosin might be the major antioxidant compound in BYF.
慢性阻塞性肺疾病（COPD）发病率和死亡率高，对全球人类健康构成威胁。大量临床试验证实，中药补肺益肾方（BYF）可通过减轻氧化应激介导的炎症来缓解COPD症状。然而，BYF的活性成分尚不清楚。我们开发了一种高效的超高效液相色谱Q-萃取轨道阱质谱法，通过基于1,1-二苯基-2-三硝基苯肼（DPPH）-液相色谱-质谱的离线筛选策略来鉴定BYF的成分并确定其抗氧化特性。在BYF提取物中总共鉴定出189种化合物，包括83种黄酮类化合物、24种木脂素、20种生物碱、15种皂苷、11种萜类化合物、10种糖类、8种脂质、7种有机酸、2种香豆素、2种氨基酸和7种其他化合物。其中，发现79种化合物具有潜在的抗氧化活性。验证表明，迷迭香酸和毛蕊异黄酮的自由基清除活性与阳性对照相似（DPPH IC分别为25.72±1.02和147.23±25.12μg/mL）。此外，口服BYF后血清中毛蕊异黄酮含量较高，表明毛蕊异黄酮可能是BYF中的主要抗氧化化合物。
