College of Landscape Architecture, Beijing Forestry University, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing, 100083, China; Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Phytochemistry. 2020 Jun;174:112331. doi: 10.1016/j.phytochem.2020.112331. Epub 2020 Mar 5.
Paeonia Radix Rubra (PRR) is a very common traditional Chinese medicine (TCM). The roots of Paeonia lactiflora and Paeonia anomala subsp. veitchii are used for the production of PRR. However, other species of section Paeonia in China are also used to produce PRR. The roots of section Paeonia from 20 populations of seven species and two subspecies in China were analyzed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF- MS). A total of 21 metabolites were identified, including nine monoterpene glycosides, seven tannins, three phenols, one paeonol and one flavonoid. There were significant differences in the composition and content of metabolites among different populations. The relative contents of monoterpene glycosides and tannins were generally higher in most samples. Cluster analysis showed that 20 populations could be divided into four groups. Among them, the populations of P. lactiflora and Paeonia mairei were clustered together in one group. The content of paeoniflorin in P. lactiflora was high (>22.20 mg g, dry weight, the same below), and the content of lactiflorin in P. mairei (>17.50 mg g) was significantly higher than in other species (<2.30 mg g). The monoterpene glycoside content in P. mairei (>51.60 mg g) was significantly higher than in other species (<43.40 mg g), suggesting that it could be useful medicinal germplasm for the development of monoterpene glycosides. In addition, some populations of Paeonia emodi, Paeonia sterniana and P. mairei may also be used as potential germplasm resources for use in PRR. Genetic and environmental factors resulted in differences in the composition and content of metabolites in different populations of the same species. Therefore, it is necessary to carefully consider the origin of Paeonia germplasm when selecting medicinal materials to be used as resources for the production of PRR.
赤芍（PRR）是一种非常常见的中药。白芍和川赤芍的根用于生产 PRR。然而，中国的牡丹属其他物种也用于生产 PRR。对来自中国 7 个种和 2 个亚种的 20 个种群的牡丹根进行了高效液相色谱-四极杆飞行时间质谱联用（HPLC-Q-TOF-MS）分析。共鉴定出 21 种代谢产物，包括 9 种单萜糖苷、7 种鞣质、3 种酚类、1 种丹皮酚和 1 种黄酮类化合物。不同种群之间代谢产物的组成和含量存在显著差异。大多数样品中单萜糖苷和鞣质的相对含量普遍较高。聚类分析表明，20 个种群可分为 4 组。其中，白芍和牡丹的种群聚为一组。白芍中芍药苷的含量较高（>22.20mg/g，下同），牡丹中丹皮酚的含量（>17.50mg/g）明显高于其他种（<2.30mg/g）。牡丹中单萜糖苷的含量（>51.60mg/g）明显高于其他种（<43.40mg/g），表明其可能是开发单萜糖苷的有用药用种质。此外，一些丹皮、川赤芍和牡丹种群也可能是 PRR 生产的潜在种质资源。遗传和环境因素导致同一物种不同种群的代谢产物组成和含量存在差异。因此，在选择药用材料作为 PRR 生产资源时，有必要仔细考虑牡丹种质的来源。
