von Bodman S B, Shaw P D
Plasmid. 1987 May;17(3):240-7. doi: 10.1016/0147-619x(87)90032-1.
Thirty isolates of Pseudomonas syringae pv. tabaci, pv. angulata (pathogens on tobacco), pv. coronafaciens, and pv. striafaciens (pathogens on oats) were examined for plasmid DNAs. The strains were obtained from plants throughout the world, some over 50 years ago. Of the 22 tobacco pathogens, 16 contain predominantly one type of plasmid, the pJP27.00 type. The remaining six tobacco-specific strains do not harbor detectable plasmids. The oat pathogens contain one, two, or three plasmids. DNA homology studies indicate that the plasmid DNAs are highly conserved. More importantly, the plasmids harbored by strains isolated from one host plant are conserved most stringently; e.g., the plasmids from the tobacco pathogens are, with one exception, indistinguishable by restriction endonuclease digestion and Southern hybridization. There is also extensive homology among plasmids indigenous to the oat-specific P. syringae pv. coronafaciens and pv. striafaciens strains.
对30株丁香假单胞菌烟草致病变种、烟草角斑致病变种（烟草病原菌）、燕麦晕斑致病变种和燕麦条纹致病变种（燕麦病原菌）进行了质粒DNA检测。这些菌株取自世界各地的植物，有些是50多年前采集的。在22株烟草病原菌中，16株主要含有一种类型的质粒，即pJP27.00型。其余6株烟草特异性菌株未检测到质粒。燕麦病原菌含有1个、2个或3个质粒。DNA同源性研究表明，质粒DNA高度保守。更重要的是，从一种寄主植物分离的菌株所携带的质粒最为严格地保守；例如，烟草病原菌的质粒，除了一个例外，经限制性内切酶消化和Southern杂交无法区分。燕麦特异性丁香假单胞菌燕麦晕斑致病变种和燕麦条纹致病变种菌株所携带的质粒之间也存在广泛的同源性。
