Department of Electrical and Computer Engineering, Boston University, Boston, Massachusetts 02215, United States.
Photonics Center, Boston University, Boston, Massachusetts 02215, United States.
J Phys Chem B. 2022 Nov 3;126(43):8597-8613. doi: 10.1021/acs.jpcb.2c05827. Epub 2022 Oct 26.
Midinfrared photothermal (MIP) microscopy, also called optical photothermal infrared (O-PTIR) microscopy, is an emerging tool for bond-selective chemical imaging of living biological and material samples. In MIP microscopy, a visible probe beam detects the photothermal-based contrast induced by a vibrational absorption. With submicron spatial resolution, high spectral fidelity, and reduced water absorption background, MIP microscopy has overcome the limitations in infrared chemical imaging methods. In this review, we summarize the basic principle of MIP microscopy, the different origins of MIP contrasts, and recent technology development that pushed the resolution, speed, and sensitivity of MIP imaging to a new stage. We further emphasize its broad applications in life science and material characterization, and provide a perspective of future technical advances.
中红外光热（MIP）显微镜，也称为光学光热红外（O-PTIR）显微镜，是一种新兴的工具，用于对活的生物和材料样品进行键选择性化学成像。在 MIP 显微镜中，可见探测光束检测由振动吸收引起的基于光热的对比度。MIP 显微镜具有亚微米级的空间分辨率、高光谱保真度和减少的水吸收背景，克服了红外化学成像方法的局限性。在这篇综述中，我们总结了 MIP 显微镜的基本原理、MIP 对比度的不同来源，以及推动 MIP 成像分辨率、速度和灵敏度达到新水平的最新技术发展。我们进一步强调了它在生命科学和材料特性表征中的广泛应用，并对未来的技术进步提供了展望。
