Department of Clinical Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Microbiol Spectr. 2022 Dec 21;10(6):e0201822. doi: 10.1128/spectrum.02018-22. Epub 2022 Oct 26.
Recently, the incidence of diseases caused by nontuberculous mycobacteria (NTM) has been increasing worldwide, especially in immunocompromised patients and those with potential chronic lung disease. Vitek MS v3.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid and reliable method for identifying mycobacteria in clinical laboratories. This study aimed to evaluate the performance of Vitek MS v3.0 by isolating NTM directly from automated liquid medium systems using patient samples. A total of 855 Mycobacterium growth indicator tube (MGIT)-positive liquid cultures were investigated. Among them, 658 (77.0%) liquid cultures were correctly identified to the species, group, or complex level, 192 (23.0%) resulted in no identification, and 5 (0.6%) were misidentified at the species level. DNA sequencing identified 855 NTM isolates from liquid cultures, comprising 316 isolates of rapidly growing mycobacteria (RGM) and 539 isolates of slow-growing mycobacteria (SGM). Using the Vitek MS system, the RGM integral identification rate (276/316 [87.34%]) was higher than the SGM rate (381/539 [70.69%]) ( < 0.01). It was also higher than the SGM rate for all MGIT report-positive periods. These results indicate that the Vitek MS v3.0 system can rapidly identify NTM species from liquid cultures. Further validation using molecular techniques is required. Rapid and accurate identification of nontuberculous mycobacteria (NTM) is essential for diagnosis, appropriate therapy, and infection control. Vitek MS v3.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid and reliable method for identifying mycobacteria in clinical laboratories. This study reported a clinical validation of the Vitek MS V3.0 system for identification of NTM isolates from 855 MGIT-positive liquid cultures which contained relatively large NTM types. Vitek MS v3.0 showed a promising rate for identification NTM isolates in positive liquid cultures. Vitek MS v3.0 had a better performance with RGM than with SGM. Vitek MS v3.0 results included "unidentified" or "misidentified" NTM isolates, which would also serve as an important reference for future optimization of this system. Vitek MS v3.0 represented a valuable technique for NTM identification from positive liquid cultures.
最近，非结核分枝杆菌（NTM）引起的疾病的发病率在全球范围内呈上升趋势，尤其是在免疫功能低下的患者和有潜在慢性肺部疾病的患者中。Vitek MS v3.0 基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成为临床实验室中快速可靠的分枝杆菌鉴定方法。本研究旨在通过直接从自动化液体培养基系统中分离 NTM 来评估 Vitek MS v3.0 的性能，使用患者样本。共调查了 855 例分枝杆菌生长指示管（MGIT）阳性液体培养物。其中，658 例（77.0%）液体培养物在种、组或复合物水平上得到正确鉴定，192 例（23.0%）未鉴定，5 例（0.6%）在种水平上误鉴定。DNA 测序鉴定了 855 例液体培养物中的 NTM 分离株，包括 316 株快速生长分枝杆菌（RGM）和 539 株缓慢生长分枝杆菌（SGM）。使用 Vitek MS 系统，RGM 整体鉴定率（276/316 [87.34%]）高于 SGM 率（381/539 [70.69%]）（ < 0.01）。它也高于所有 MGIT 报告阳性期的 SGM 率。这些结果表明，Vitek MS v3.0 系统可快速鉴定液体培养物中的 NTM 种。需要进一步使用分子技术进行验证。
快速准确地鉴定非结核分枝杆菌（NTM）对于诊断、适当的治疗和感染控制至关重要。Vitek MS v3.0 基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成为临床实验室中快速可靠的分枝杆菌鉴定方法。本研究报告了 Vitek MS V3.0 系统对 855 例 MGIT 阳性液体培养物中 NTM 分离株的临床验证，这些培养物中包含相对较大的 NTM 类型。Vitek MS v3.0 对阳性液体培养物中的 NTM 分离株显示出有希望的鉴定率。Vitek MS v3.0 对 RGM 的鉴定性能优于 SGM。Vitek MS v3.0 的结果包括“未鉴定”或“误鉴定”的 NTM 分离株，这也将为该系统的未来优化提供重要参考。Vitek MS v3.0 代表了从阳性液体培养物中鉴定 NTM 的一种有价值的技术。
