Kehrmann Jan, Schoerding Ann-Kathrin, Murali Roshni, Wessel Sarah, Koehling Hedda Luise, Mosel Frank, Buer Jan
Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Diagn Microbiol Infect Dis. 2016 Jan;84(1):43-47. doi: 10.1016/j.diagmicrobio.2015.10.007. Epub 2015 Oct 9.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is a fast and inexpensive method for bacterial identification. The aim of this study was to analyze the performance of Vitek MS in identifying 160 nontuberculous mycobacterial isolates of 24 species from Lowenstein-Jensen solid medium and BACTEC MGIT 960 liquid medium using a bead-based method. The system correctly identified 76.9% of the isolates (123 of 160) cultivated on solid medium and 76.9% (123 of 160) of positive liquid cultures. None of the isolates included in the study was misidentified. Although the overall performance of Vitek MS with the SARAMIS 4.12 database was comparable in identifying mycobacterial species grown on solid medium and in liquid medium, the identification rate varied notably between the various species analyzed, which currently limits the utility for identification in routine diagnostics for some species.
基质辅助激光解吸/电离飞行时间质谱是一种快速且经济的细菌鉴定方法。本研究的目的是使用基于磁珠的方法分析Vitek MS在鉴定来自罗氏固体培养基和BACTEC MGIT 960液体培养基的24种160株非结核分枝杆菌分离株方面的性能。该系统正确鉴定了在固体培养基上培养的76.9%的分离株（160株中的123株）以及76.9%（160株中的123株）的阳性液体培养物。研究中纳入的分离株均未被错误鉴定。尽管使用SARAMIS 4.12数据库的Vitek MS在鉴定固体培养基和液体培养基上生长的分枝杆菌物种方面的总体性能相当，但在分析的不同物种之间鉴定率差异显著，这目前限制了其在某些物种常规诊断鉴定中的实用性。
