Department of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen 518052, PR China.
Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Bio-medical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, PR China.
J Med Microbiol. 2022 Oct;71(10). doi: 10.1099/jmm.0.001587.
is a major cause of chronic diseases and biofilm formation is a contributing factor. 20S-ginsenoside Rg3 (Rg3) is a natural product extracted from the traditional Chinese medicine red ginseng. The effects of Rg3 on biofilm formation and haemolytic activity as well as its antibacterial mechanism against have not been reported. This study aimed to investigate the effects of Rg3 on biofilm formation and haemolytic activity as well as its antibacterial action against clinical isolates. The effect of Rg3 on biofilm formation of clinical isolates was studied by crystal violet staining. Haemolytic activity analysis was carried out. Furthermore, the influence of Rg3 on the proteome profile of was studied by quantitative proteomics to clarify the mechanism underlying its antibacterial action and further verified by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Rg3 significantly inhibited biofilm formation and haemolytic activity in clinical isolates. A total of 63 with >1.5-fold changes in expression were identified, including 34 upregulated proteins and 29 downregulated proteins. Based on bioinformatics analysis, the expression of several virulence factors and biofilm-related proteins, containing CopZ, CspA, SasG, SaeR/SaeS two-component system and SaeR/SaeS-regulated proteins, including leukocidin-like protein 2, immunoglobulin-binding protein G (Sbi) and fibrinogen-binding protein, in the s of the Rg3-treated group was downregulated. RT-qPCR confirmed that Rg3 inhibited the regulation of SaeR/SaeS and decreased the transcriptional levels of the biofilm-related genes C, and . Rg3 reduces the formation of biofilm by reducing cell adhesion and aggregation. Further, Rg3 can inhibit the SaeR/SaeS two-component system, which acts as a crucial signal transduction system for the anti-virulence activity of Rg3 against clinical isolates.
是慢性疾病的主要原因，生物膜的形成是一个促成因素。20S-人参皂苷 Rg3（Rg3）是从传统中药红参中提取的天然产物。Rg3 对生物膜形成和溶血活性的影响及其对 的抗菌机制尚未报道。本研究旨在探讨 Rg3 对生物膜形成和溶血活性的影响及其对临床 分离株的抗菌作用。通过结晶紫染色研究 Rg3 对临床 分离株生物膜形成的影响。进行溶血活性分析。此外，通过定量蛋白质组学研究 Rg3 对 蛋白质组谱的影响，以阐明其抗菌作用的机制，并通过逆转录定量实时聚合酶链反应（RT-qPCR）进一步验证。Rg3 显著抑制了临床 分离株的生物膜形成和溶血活性。共鉴定出 63 个表达水平变化>1.5 倍的蛋白，包括 34 个上调蛋白和 29 个下调蛋白。基于生物信息学分析，下调了一些毒力因子和生物膜相关蛋白的表达，包括 CopZ、CspA、SasG、SaeR/SaeS 双组分系统和 SaeR/SaeS 调节蛋白，包括白细胞毒素样蛋白 2、免疫球蛋白结合蛋白 G（Sbi）和纤维蛋白原结合蛋白。RT-qPCR 证实 Rg3 抑制了 SaeR/SaeS 的调节，降低了生物膜相关基因 C、 和 的转录水平。Rg3 通过减少细胞黏附和聚集来减少生物膜的形成。此外，Rg3 可以抑制 SaeR/SaeS 双组分系统，该系统作为 Rg3 对临床 分离株抗毒力活性的关键信号转导系统。
