School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005598.
Three extremely halophilic archaeal strains (LT55, SQT-29-1 and WLHS5) were isolated from Gobi saline soil and a salt lake, China. These strains were most related to the genera and (92.6-95.1 % similarities), and showed low similarities with other genera within the family based on 16S rRNA genes. Phylogenomic analysis confirmed that the three strains formed a distinct clade separated from the related genera and , which indicated that they may represent a novel genus of the family . The average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH) and average amino acid identity (AAI) values among the three strains were no more than 87, 34 and 85 %, respectively, much lower than the threshold values for species demarcation. The major phospholipids of the three strains were phosphatidic acid (PA), phosphatidylglycerol (PG) and phosphatidylglycerol phosphate methyl ester (PGP-Me). The glycolipid profiles of the three strains were diverse; sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S-DGD) were found in strains LT55 and WLHS5, while mannosyl glucosyl diether (DGD-1) and S-DGD-1 in strain SQT-29-1. The combination of phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strains WLHS5 (=CGMCC 1.13781 = JCM 33558), SQT-29-1 (=CGMCC 1.16065 = JCM 33554) and LT55 (=CGMCC 1.15188 = JCM 30838) represent three novel species of a new genus within the family , for which the names, gen. nov., sp. nov., sp. nov. and sp. nov., are proposed. Genome-based classification of genera and revealed that should be transferred to the genus as comb. nov. and as a heterotypic synonym of Liu . 2015.
三株极端嗜盐古菌（LT55、SQT-29-1 和 WLHS5）从中国戈壁盐土和盐湖中分离得到。这些菌株与属（相似度 92.6-95.1%）最为相关，而与科内其他属的相似度较低，基于 16S rRNA 基因。系统发育基因组分析证实，这三株菌形成了一个与相关属和分离的独特分支，表明它们可能代表科内的一个新属。三株菌之间的平均核苷酸同一性（ANI）、体外 DNA-DNA 杂交（DDH）和平均氨基酸同一性（AAI）值均不超过 87%、34%和 85%，远低于种的划分阈值。三株菌的主要磷脂为磷脂酸（PA）、磷脂酰甘油（PG）和磷脂酰甘油磷酸甲醚（PGP-Me）。三株菌的糖脂图谱各不相同；LT55 和 WLHS5 株中存在硫酸甘露糖葡糖基二醚（S-DGD-1）和二硫酸甘露糖葡糖基二醚（S-DGD），而 SQT-29-1 株中存在甘露糖葡糖基二醚（DGD-1）和 S-DGD-1。表型、化学分类、系统发育和基因组分析相结合表明，WLHS5 株（=CGMCC 1.13781=JCM 33558）、SQT-29-1 株（=CGMCC 1.16065=JCM 33554）和 LT55 株（=CGMCC 1.15188=JCM 30838）代表了科内一个新属的三个新种，分别命名为，属，新属，种，新种和，种，新种。基于基因组的分类，将属和转移到属中，作为组合新属，和作为的异型同义词，刘。2015 年。
