Ferguson A V, Kasting N W
Regul Pept. 1987 Aug 3;18(2):93-100. doi: 10.1016/0167-0115(87)90039-5.
The effects of activation of subfornical organ (SFO) efferents on plasma oxytocin concentrations were examined in conscious freely moving male Sprague-Dawley rats. Blood samples were obtained through chronically implanted atrial catheters and SFO efferents were activated electrically using chronically implanted bipolar stimulating electrodes. Hormone concentrations were measured by radioimmunoassay, and experimental animals were assigned to one of 3 experimental groups according to histologically verified anatomical locations of stimulating electrodes in either the SFO, the hippocampal commissure (HC), or the medial septum (MS). Electrical stimulation in the SFO resulted in increased plasma concentrations of oxytocin from control values of 2.54 +/- 0.9 pg/ml, to a post-stimulation level of 65.6 +/- 27.0 pg/ml. In contrast, stimulation in immediately adjacent structures including HC and MS was found to be without effect on plasma concentrations of oxytocin. These studies provide the first definitive evidence that SFO efferents may play a significant role in controlling the secretion of oxytocin from the posterior pituitary.
在清醒自由活动的雄性Sprague-Dawley大鼠中，研究了穹窿下器（SFO）传出神经激活对血浆催产素浓度的影响。通过长期植入的心房导管采集血样，使用长期植入的双极刺激电极电激活SFO传出神经。采用放射免疫分析法测定激素浓度，并根据组织学验证的刺激电极在SFO、海马连合（HC）或内侧隔区（MS）的解剖位置，将实验动物分为3个实验组之一。SFO的电刺激导致血浆催产素浓度从2.54±0.9 pg/ml的对照值增加到刺激后的65.6±27.0 pg/ml。相比之下，发现刺激紧邻结构（包括HC和MS）对血浆催产素浓度没有影响。这些研究提供了首个确凿证据，表明SFO传出神经可能在控制垂体后叶催产素分泌中发挥重要作用。
