Gaudet Camille, Solecki Lauriana, Mathéaud Bastien, Odet Stephane, Meyer Christophe, Louvrier Aurélien, Gindraux Florelle
Service de Chirurgie Maxillo-Faciale, Stomatologie et Odontologie Hospitalière, CHU Besançon, F-25000 Besançon, France.
Service d'Ophtalmologie, CHU Besançon, F-25000 Besançon, France.
Biomedicines. 2022 Oct 1;10(10):2456. doi: 10.3390/biomedicines10102456.
We questioned the relevance of evaluating residual cell viability in human amniotic membrane (hAM) after its cryopreservation since cell survival is controversial and its ability to act as a matrix (including the presence of growth factors and cytokines) appears to be most important for tissue regeneration purposes. We also discussed the usefulness of osteodifferentiating amniotic cells in whole hAM for bone repair applications. We have evidence that determining residual cell viability after cryopreservation and hAM osteodifferentiation is not justified.
我们质疑了在人羊膜（hAM）冷冻保存后评估其残余细胞活力的相关性，因为细胞存活存在争议，并且就组织再生目的而言，其作为基质的能力（包括生长因子和细胞因子的存在）似乎最为重要。我们还讨论了在整个hAM中使羊膜细胞骨分化用于骨修复应用的效用。我们有证据表明，在冷冻保存和hAM骨分化后确定残余细胞活力是不合理的。
