Sedgwick A D, Dawson J, Lees P
Res Vet Sci. 1987 Jul;43(1):55-8.
Subpopulations of equine leucocytes, polymorphonuclear and mononuclear cells, were separated from whole blood on a discontinuous Percoll gradient and used in studies of chemokinesis and chemotaxis. Polymorphonuclear cells responded to the chemo-attractant properties of zymosan-activated plasma in Boyden chamber and agarose microdroplet assays but they responded only slightly (Boyden chamber) or not at all (agarose microdroplet) to the peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP). Equine mononuclear cell movement was increased by FMLP in both assay systems and these cells also responded to zymosan activated plasma in the Boyden chamber assay but not in the agarose microdroplet. It is concluded that factors controlling equine polymorphonuclear and mononuclear cell movements into inflammatory exudates may differ.
马白细胞的亚群，即多形核细胞和单核细胞，通过不连续的Percoll梯度从全血中分离出来，并用于趋化作用和趋化性的研究。在Boyden小室和琼脂糖微滴试验中，多形核细胞对酵母聚糖激活的血浆的化学吸引特性有反应，但它们对肽N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）仅产生轻微反应（Boyden小室）或根本没有反应（琼脂糖微滴）。在这两种试验系统中，FMLP均可增加马单核细胞的移动，并且这些细胞在Boyden小室试验中也对酵母聚糖激活的血浆有反应，但在琼脂糖微滴试验中则无反应。得出的结论是，控制马多形核细胞和单核细胞向炎症渗出物移动的因素可能不同。
