Camp C J, Leid R W
Am J Vet Res. 1982 Mar;43(3):397-401.
A method for the isolation of equine neutrophils was developed using metrizamide cushions. A purity of greater than 95% was routinely obtained with greater than 90% viability. These cells were radiolabeled and tested for their chemotactic response in Boyden chambers to zymosan-activated equine serum, the partially purified equine complement component C5a, and formyl-L-methionyl-L-leucyl-L-phenylalanine. The time and ionic requirements for chemotaxis of radiolabeled equine neutrophils were investigated and maximal movement was observed at 2 hours' incubation and 1.0 mM Ca and 0.5 mM Mg. Dinitrophenol in a concentration range of 10(-2) to 10(-6) M also inhibited cell movement. A Zigmond-Hirsch checkerboard assay demonstrated the pronounced chemotactic activity of zymosan-activated equine serum and the partially purified equine C5a. The equine neutrophil did not respond to formyl-L-methionyl-L-leucyl-L-phenylalanine.
利用甲泛葡胺垫层开发了一种分离马中性粒细胞的方法。常规获得的纯度大于95%，活力大于90%。这些细胞经放射性标记，并在博伊登小室中测试它们对酵母聚糖激活的马血清、部分纯化的马补体成分C5a和甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸的趋化反应。研究了放射性标记的马中性粒细胞趋化作用的时间和离子需求，在孵育2小时、1.0 mM钙和0.5 mM镁时观察到最大运动。浓度范围为10(-2)至10(-6) M的二硝基苯酚也抑制细胞运动。齐格蒙德-赫希棋盘试验证明了酵母聚糖激活的马血清和部分纯化的马C5a具有显著的趋化活性。马中性粒细胞对甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸无反应。
