• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-29a 对巩膜成纤维细胞和视网膜色素上皮细胞基质金属蛋白酶-2 分泌的调控。

Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a.

机构信息

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Departments of Ophthalmology and Pathology, New York Eye and Ear Infirmary of Mount Sinai, New York, NY, USA.

出版信息

Biomed Res Int. 2017;2017:2647879. doi: 10.1155/2017/2647879. Epub 2017 May 29.

DOI:10.1155/2017/2647879
PMID:28630860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5467432/
Abstract

PURPOSE

To identify an effective method to prevent myopia progression by characterizing the regulation of matrix metalloproteinase- (MMP-) 2 expression and its secretion from scleral fibroblasts and retinal pigment epithelium (RPE) cells by miR-29a.

METHODS

The effects of miR-29a on the growth of scleral fibroblasts and RPE cells were assessed using the cell counting kit-8. The changes in MMP-2 mRNA levels in scleral fibroblasts and RPE cells after transfection with miR-29a mimics or inhibitor were measured by quantitative PCR. Enzyme-linked immunosorbent assays were used to determine the changes in MMP-2 secretion from scleral fibroblasts and RPE cells after transfection with miR-29a mimics or inhibitor.

RESULTS

The miR-29a mimics or inhibitor did not significantly alter the growth of scleral fibroblasts or RPE cells at 24, 48, or 72 hours after transfection. MMP-2 mRNA levels were significantly decreased in scleral fibroblasts and RPE cells transfected with the miR-29a mimics. The secretion of MMP-2 by scleral fibroblasts and RPE cells was significantly decreased in cells transfected with the miR-29a mimics.

CONCLUSIONS

Suppression of scleral fibroblast and RPE cell expression and secretion of MMP-2 by miR-29a can be used as a therapeutic target for the prevention and treatment of myopia.

摘要

目的

通过鉴定 miR-29a 对基质金属蛋白酶-2（MMP-2）表达及其在巩膜成纤维细胞和视网膜色素上皮（RPE）细胞中分泌的调控作用，找到一种有效预防近视进展的方法。

方法

通过细胞计数试剂盒-8 评估 miR-29a 对巩膜成纤维细胞和 RPE 细胞生长的影响。用定量 PCR 检测转染 miR-29a 模拟物或抑制剂后巩膜成纤维细胞和 RPE 细胞中 MMP-2 mRNA 水平的变化。用酶联免疫吸附试验检测转染 miR-29a 模拟物或抑制剂后巩膜成纤维细胞和 RPE 细胞中 MMP-2 分泌的变化。

结果

转染 miR-29a 模拟物或抑制剂 24、48 或 72 小时后，对巩膜成纤维细胞或 RPE 细胞的生长没有明显影响。转染 miR-29a 模拟物后，巩膜成纤维细胞和 RPE 细胞中 MMP-2 mRNA 水平显著降低。转染 miR-29a 模拟物后，巩膜成纤维细胞和 RPE 细胞中 MMP-2 的分泌明显减少。

结论

miR-29a 抑制巩膜成纤维细胞和 RPE 细胞 MMP-2 的表达和分泌，可作为预防和治疗近视的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/47d7ad4dd206/BMRI2017-2647879.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/7a5063bd507e/BMRI2017-2647879.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/e474a84428db/BMRI2017-2647879.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/e68b2a5f77f0/BMRI2017-2647879.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/590611c7f245/BMRI2017-2647879.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/47d7ad4dd206/BMRI2017-2647879.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/7a5063bd507e/BMRI2017-2647879.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/e474a84428db/BMRI2017-2647879.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/e68b2a5f77f0/BMRI2017-2647879.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/590611c7f245/BMRI2017-2647879.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d4/5467432/47d7ad4dd206/BMRI2017-2647879.005.jpg

相似文献

1
Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a.miR-29a 对巩膜成纤维细胞和视网膜色素上皮细胞基质金属蛋白酶-2 分泌的调控。
Biomed Res Int. 2017;2017:2647879. doi: 10.1155/2017/2647879. Epub 2017 May 29.
2
A Potential Research Target for Scleral Remodeling: Effect of MiR-29a on Scleral Fibroblasts.巩膜重塑的潜在研究靶点：miR-29a 对巩膜成纤维细胞的作用。
Ophthalmic Res. 2022;65(5):566-574. doi: 10.1159/000525189. Epub 2022 May 23.
3
Suppression of type I collagen in human scleral fibroblasts treated with extremely low-frequency electromagnetic fields.极低频电磁场处理后人巩膜成纤维细胞中I型胶原蛋白的抑制作用
Mol Vis. 2013 Apr 12;19:885-93. Print 2013.
4
Genipin inhibits the scleral expression of miR-29 and MMP2 and promotes COL1A1 expression in myopic eyes of guinea pigs.京尼平抑制豚鼠近视眼中 miR-29 和 MMP2 的巩膜表达并促进 COL1A1 的表达。
Graefes Arch Clin Exp Ophthalmol. 2020 May;258(5):1031-1038. doi: 10.1007/s00417-020-04634-7. Epub 2020 Mar 3.
5
Roles of NFκB-miR-29s-MMP-2 circuitry in experimental choroidal neovascularization.NFκB-微小RNA-29s-基质金属蛋白酶-2信号通路在实验性脉络膜新生血管形成中的作用
J Neuroinflammation. 2014 May 15;11:88. doi: 10.1186/1742-2094-11-88.
6
GABAergic agents modify the response of chick scleral fibroblasts to myopic and hyperopic eye cup tissues.GABA 能药物改变小鸡巩膜成纤维细胞对近视和远视眼球杯组织的反应。
Curr Eye Res. 2014 Feb;39(2):172-87. doi: 10.3109/02713683.2013.834941. Epub 2013 Sep 27.
7
MiR-29a reduces TIMP-1 production by dermal fibroblasts via targeting TGF-β activated kinase 1 binding protein 1, implications for systemic sclerosis.微小RNA-29a通过靶向转化生长因子-β激活激酶1结合蛋白1降低真皮成纤维细胞中金属蛋白酶组织抑制因子-1的产生，对系统性硬化症具有重要意义。
PLoS One. 2014 Dec 30;9(12):e115596. doi: 10.1371/journal.pone.0115596. eCollection 2014.
8
Scleral cell growth is influenced by retinal pigment epithelium in vitro.体外培养时，巩膜细胞生长受视网膜色素上皮影响。
Graefes Arch Clin Exp Ophthalmol. 1994 Sep;232(9):545-52. doi: 10.1007/BF00181998.
9
Brain-derived neurotrophic factor is a novel target gene of the has-miR-183/96/182 cluster in retinal pigment epithelial cells following visible light exposure.脑源性神经营养因子是视网膜色素上皮细胞在可见光照射后has-miR-183/96/182簇的一个新的靶基因。
Mol Med Rep. 2015 Aug;12(2):2793-9. doi: 10.3892/mmr.2015.3736. Epub 2015 May 6.
10
Comparative effects of posterior eye cup tissues from myopic and hyperopic chick eyes on cultured scleral fibroblasts.近视和远视鸡眼球后杯组织对培养巩膜成纤维细胞的比较作用。
Exp Eye Res. 2013 Feb;107:11-20. doi: 10.1016/j.exer.2012.11.005. Epub 2012 Nov 28.

引用本文的文献

1
Research Progress of Epigenetic Modifications in Myopia.近视中表观遗传修饰的研究进展
Int J Med Sci. 2025 Jun 23;22(12):3084-3100. doi: 10.7150/ijms.110640. eCollection 2025.
2
HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.组蛋白去乙酰化酶1参与中风发病机制中的神经炎症和血脑屏障损伤。
J Inflamm Res. 2023 Sep 18;16:4103-4116. doi: 10.2147/JIR.S416239. eCollection 2023.
3
Identification of miR-671-5p and Its Related Pathways as General Mechanisms of Both Form-Deprivation and Lens-Induced Myopia in Mice.

本文引用的文献

1
Retinal pigment epithelium cell density in relationship to axial length in human eyes.人眼中视网膜色素上皮细胞密度与眼轴长度的关系
Acta Ophthalmol. 2017 Feb;95(1):e22-e28. doi: 10.1111/aos.13188. Epub 2016 Aug 22.
2
Genetic variants in MiR-29a associated with high myopia.与高度近视相关的MiR-29a基因变异
Ophthalmic Genet. 2016 Dec;37(4):456-458. doi: 10.3109/13816810.2015.1101776. Epub 2016 Feb 17.
3
Axial eye growth and refractive error development can be modified by exposing the peripheral retina to relative myopic or hyperopic defocus.
鉴定miR-671-5p及其相关通路作为小鼠形觉剥夺性近视和晶状体诱导性近视的共同机制
Curr Issues Mol Biol. 2023 Mar 2;45(3):2060-2072. doi: 10.3390/cimb45030132.
4
Differential methylation of microRNA encoding genes may contribute to high myopia.微小RNA编码基因的差异甲基化可能导致高度近视。
Front Genet. 2023 Jan 4;13:1089784. doi: 10.3389/fgene.2022.1089784. eCollection 2022.
5
The Role of Retinal Dysfunction in Myopia Development.视网膜功能障碍在近视发展中的作用。
Cell Mol Neurobiol. 2023 Jul;43(5):1905-1930. doi: 10.1007/s10571-022-01309-1. Epub 2022 Nov 24.
6
The Effect of Citicoline on the Expression of Matrix Metalloproteinase-2 (MMP-2), Transforming Growth Factor-β1 (TGF-β1), and Ki-67, and on the Thickness of Scleral Tissue of Rat Myopia Model.胞磷胆碱对大鼠近视模型基质金属蛋白酶-2（MMP-2）、转化生长因子-β1（TGF-β1）和Ki-67表达及巩膜组织厚度的影响
Biomedicines. 2022 Oct 17;10(10):2600. doi: 10.3390/biomedicines10102600.
7
Scleral remodeling in myopia development.近视发展中的巩膜重塑。
Int J Ophthalmol. 2022 Mar 18;15(3):510-514. doi: 10.18240/ijo.2022.03.21. eCollection 2022.
8
Epigenetics in blood-brain barrier disruption.血脑屏障破坏中的表观遗传学
Fluids Barriers CNS. 2021 Apr 6;18(1):17. doi: 10.1186/s12987-021-00250-7.
9
miR-200-3p suppresses cell proliferation and reduces apoptosis in diabetic retinopathy via blocking the TGF-β2/Smad pathway.miR-200-3p 通过阻断 TGF-β2/Smad 通路抑制糖尿病视网膜病变中的细胞增殖并减少细胞凋亡。
Biosci Rep. 2020 Nov 27;40(11). doi: 10.1042/BSR20201545.
10
miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with HO.HO 培养的 ARPE-19 细胞来源的小细胞外囊泡中 miR302a 和 122 失调。
Sci Rep. 2019 Nov 29;9(1):17954. doi: 10.1038/s41598-019-54373-x.
通过使周边视网膜暴露于相对近视或远视性离焦状态，可改变眼轴生长和屈光不正的发展。
Invest Ophthalmol Vis Sci. 2014 Sep 4;55(10):6765-73. doi: 10.1167/iovs.14-14524.
4
Roles of NFκB-miR-29s-MMP-2 circuitry in experimental choroidal neovascularization.NFκB-微小RNA-29s-基质金属蛋白酶-2信号通路在实验性脉络膜新生血管形成中的作用
J Neuroinflammation. 2014 May 15;11:88. doi: 10.1186/1742-2094-11-88.
5
Effects of hepatocyte growth factor on MMP-2 expression in scleral fibroblasts from a guinea pig myopia model.肝细胞生长因子对豚鼠近视模型巩膜成纤维细胞中MMP-2表达的影响。
Int J Ophthalmol. 2014 Apr 18;7(2):239-44. doi: 10.3980/j.issn.2222-3959.2014.02.09. eCollection 2014.
6
Myopia control in children through refractive therapy gas permeable contact lenses: is it for real?儿童近视控制的硬性透气性角膜接触镜疗法：真能实现吗？
Am J Ophthalmol. 2013 Dec;156(6):1076-1081.e1. doi: 10.1016/j.ajo.2013.04.039.
7
MicroRNA-29a upregulates MMP2 in oral squamous cell carcinoma to promote cancer invasion and anti-apoptosis.微小 RNA-29a 通过上调 MMP2 促进口腔鳞状细胞癌侵袭和抗凋亡。
Biomed Pharmacother. 2014 Feb;68(1):13-9. doi: 10.1016/j.biopha.2013.10.005. Epub 2013 Oct 18.
8
A key role of microRNA-29b for the suppression of colon cancer cell migration by American ginseng.西洋参通过 microRNA-29b 抑制结肠癌细胞迁移的关键作用。
PLoS One. 2013 Oct 9;8(10):e75034. doi: 10.1371/journal.pone.0075034. eCollection 2013.
9
MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis.miR-29c 抑制神经胶质瘤细胞增殖、迁移、侵袭和血管生成。
J Neurooncol. 2013 Nov;115(2):179-88. doi: 10.1007/s11060-013-1223-2. Epub 2013 Aug 13.
10
miRNA-29c suppresses lung cancer cell adhesion to extracellular matrix and metastasis by targeting integrin β1 and matrix metalloproteinase2 (MMP2).miRNA-29c 通过靶向整合素 β1 和基质金属蛋白酶 2（MMP2）抑制肺癌细胞与细胞外基质的黏附及转移。
PLoS One. 2013 Aug 6;8(8):e70192. doi: 10.1371/journal.pone.0070192. Print 2013.