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gH625-脂化垂体腺苷酸环化酶激活肽在帕金森病体外流体动力学模型中的神经保护作用

Neuroprotective Effects of gH625-lipoPACAP in an In Vitro Fluid Dynamic Model of Parkinson's Disease.

作者信息

Barra Teresa, Falanga Annarita, Bellavita Rosa, Pisano Jessica, Laforgia Vincenza, Prisco Marina, Galdiero Stefania, Valiante Salvatore

机构信息

Department of Biology, University of Naples Federico II, 80125 Naples, Italy.

Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.

出版信息

Biomedicines. 2022 Oct 20;10(10):2644. doi: 10.3390/biomedicines10102644.

DOI:10.3390/biomedicines10102644
PMID:36289905
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9599564/
Abstract

Parkinson's disease (PD) is an aggressive and devastating age-related disorder. Although the causes are still unclear, several factors, including genetic and environmental, are involved. Except for symptomatic drugs, there are not, to date, any real cures for PD. For this purpose, it is necessary develop a model to better study this disease. Neuroblastoma cell line, SH-SY5Y, differentiated with retinoic acid represents a good in vitro model to explore PD, since it maintains growth cells to differentiated neurons. In the present study, SH-SY5Y cells were treated with 1-methyl-4-phenylpyridinium (MPP), a neurotoxin that induces Parkinsonism, and the neuroprotective effects of pituitary adenylate cyclase-activating polypeptide (PACAP), delivered by functionalized liposomes in a blood-brain barrier fluid dynamic model, were evaluated. We demonstrated PACAP neuroprotective effects when delivered by gH625-liposome on MPP-damaged SH-SY5Y spheroids.

摘要

帕金森病（PD）是一种侵袭性且具有破坏性的与年龄相关的疾病。尽管病因仍不明确，但涉及多种因素，包括遗传和环境因素。除了对症治疗药物外，迄今为止，PD尚无真正的治愈方法。为此，有必要建立一个模型以更好地研究这种疾病。用视黄酸分化的神经母细胞瘤细胞系SH-SY5Y是探索PD的良好体外模型，因为它能使生长细胞维持为分化的神经元。在本研究中，用诱导帕金森症的神经毒素1-甲基-4-苯基吡啶鎓（MPP）处理SH-SY5Y细胞，并在血脑屏障流体动力学模型中评估功能化脂质体递送的垂体腺苷酸环化酶激活多肽（PACAP）的神经保护作用。我们证明了gH625-脂质体递送的PACAP对MPP损伤的SH-SY5Y球体具有神经保护作用。

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