Pengsart Worakamol, Kulkeaw Kasem
Graduate Study School, Faculty of Medicine Siriraj Hospital, Mahidol University, Nakhonpathom 73170, Thailand.
Siriraj Integrative Center for Neglected Parasitic Diseases, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Bioengineering (Basel). 2022 Jul 20;9(7):330. doi: 10.3390/bioengineering9070330.
A spheroid is a cell aggregate in a three-dimensional context; thereby, it recapitulates the cellular architecture in human tissue. However, the utility of spheroids as an assay for host-parasite interactions remains unexplored. This study demonstrates the potential use of neurospheroids for assessing the cytotoxicity of the life-threatening pathogenic amoeba . The neuroblastoma SH-SY5Y cells formed a spheroid in a hanging drop of culture medium. Cellular damage caused by trophozoites on human neuronal spheroids was observed using microscopic imaging and ATP detection. trophozoites rapidly caused a decrease in ATP production in the spheroid, leading to loss of neurospheroid integrity. Moreover, 3D confocal microscopy imaging revealed interactions between the trophozoites and SH-SY5Y neuronal cells in the outer layer of the neurospheroid. In conclusion, the neurospheroid allows the assessment of host cell damage in a simple and quantitative manner.
球体是三维环境中的细胞聚集体；因此，它概括了人体组织中的细胞结构。然而，球体作为宿主 - 寄生虫相互作用分析方法的实用性仍未得到探索。本研究证明了神经球体在评估危及生命的致病性变形虫细胞毒性方面的潜在用途。神经母细胞瘤SH - SY5Y细胞在培养基的悬滴中形成球体。使用显微镜成像和ATP检测观察到滋养体对人神经元球体造成的细胞损伤。滋养体迅速导致球体中ATP生成减少，导致神经球体完整性丧失。此外，三维共聚焦显微镜成像揭示了滋养体与神经球体外层的SH - SY5Y神经元细胞之间的相互作用。总之，神经球体能够以简单且定量的方式评估宿主细胞损伤。
