Siddiqui Ruqaiyyah, Boghossian Anania, Akbar Noor, Jabri Tooba, Aslam Zara, Shah Muhammad Raza, Alharbi Ahmad M, Alfahemi Hasan, Khan Naveed Ahmed
College of Arts and Sciences, American University of Sharjah, University City, Sharjah 26666, United Arab Emirates.
Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah 27272, United Arab Emirates.
Antibiotics (Basel). 2022 Sep 20;11(10):1281. doi: 10.3390/antibiotics11101281.
and are opportunistic protists, responsible for fatal central nervous system infections such as primary amoebic meningoencephalitis (PAM) and granulomatous amoebic encephalitis (GAE) with mortality rates higher than 90%. Threatening a rise in cases is the increase in temperature due to global warming. No effective treatment is currently available. Herein, nanotechnology was used to conjugate Zinc oxide with Ampicillin, Ceftrixon, Naringin, Amphotericin B, and Quericitin, and the amoebicidal activity and host cell cytotoxicity of these resulting compounds were investigated. The compounds ZnO-CD-AMPi, ZnO-CD-CFT, ZnO-CD-Nar, ZnO-CD-AMB, and ZnO-CD-QT were found to reduce viability to 35.5%, 39.6%, 52.0%, 50.8%, 35.9%, and 69.9%, respectively, and viability to 40.9%, 48.2%, 51.6%, 43.8%, and 62.4%, respectively, when compared with their corresponding controls. Furthermore, the compounds reduced -mediated and -mediated host cell death significantly. Additionally, the compounds showed limited cytotoxicity against human cells; cell toxicity was 35.5%, 36.4%, 30.9%, 36.6%, and 35.6%, respectively, for the compounds ZnO-CD-AMPi, ZnO-CD-CFT, ZnO-CD-Nar, ZnO-CD-AMB, and ZnO-CD-QT. Furthermore, the minimum inhibitory concentrations to inhibit amoeba growth by 50% were determined for and The MIC for were determined to be 69.52 µg/mL, 82.05 µg/mL, 88.16 µg/mL, 95.61 µg/mL, and 85.69 µg/mL, respectively; the MIC of the compounds for were determined to be 113.9 µg/mL, 102.3 µg/mL, 106.9 µg/mL, 146.4 µg/mL, and 129.6 µg/mL, respectively. Translational research to further develop therapies based on these compounds is urgently warranted, given the lack of effective therapies currently available against these devastating infections.
[具体名称1]和[具体名称2]是机会性原生生物，可引发致命的中枢神经系统感染，如原发性阿米巴脑膜脑炎（PAM）和肉芽肿性阿米巴脑炎（GAE），死亡率高于90%。全球变暖导致的气温上升使病例数有增加的趋势。目前尚无有效的治疗方法。在此，利用纳米技术将氧化锌与氨苄西林、头孢曲松、柚皮苷、两性霉素B和槲皮素结合，并研究了这些所得化合物的杀阿米巴活性和宿主细胞细胞毒性。发现化合物ZnO-CD-AMPi、ZnO-CD-CFT、ZnO-CD-Nar、ZnO-CD-AMB和ZnO-CD-QT与相应对照相比，分别将[某种细胞]活力降低至35.5%、39.6%、52.0%、50.8%、35.9%和6%，将[另一种细胞]活力分别降低至40.9%、48.2%、51.6%、43.8%和62.4%。此外，这些化合物显著降低了[具体介导方式1]介导的和[具体介导方式2]介导的宿主细胞死亡。此外，这些化合物对人类细胞的细胞毒性有限；化合物ZnO-CD-AMPi、ZnO-CD-CFT、ZnO-CD-Nar、ZnO-CD-AMB和ZnO-CD-QT的细胞毒性分别为35.5%、36.4%、30.9%、36.6%和35.6%。此外，还测定了[两种病原体名称]抑制50%阿米巴生长的最低抑菌浓度。[病原体1]的最低抑菌浓度分别测定为69.52 µg/mL、82.05 µg/mL、88.16 µg/mL、95.61 µg/mL和85.69 µg/mL；这些化合物对[病原体2]的最低抑菌浓度分别测定为113.9 µg/mL、102.3 µg/mL、106.9 µg/mL、146.4 µg/mL和129.6 µg/mL。鉴于目前缺乏针对这些毁灭性感染的有效疗法，迫切需要开展转化研究以进一步开发基于这些化合物的疗法。
