Makhlouf Zinb, Akbar Noor, Khan Naveed Ahmed, Shah Muhammad Raza, Alharbi Ahmad M, Alfahemi Hasan, Siddiqui Ruqaiyyah
College of Arts and Sciences, American University of Sharjah, University City, Sharjah 26666, United Arab Emirates.
Department of Clinical Sciences, College of Medicine, University of Sharjah, University City, Sharjah 27272, United Arab Emirates.
Antibiotics (Basel). 2022 Nov 30;11(12):1721. doi: 10.3390/antibiotics11121721.
is a ubiquitous free-living amoeba capable of instigating keratitis and granulomatous amoebic encephalitis in humans. Treatment remains limited and inconsistent. Accordingly, there is a pressing need for novel compounds. Nanotechnology has been gaining attention for enhancing drug delivery and reducing toxicity. Previous work has shown that various antibiotic classes displayed antiamoebic activity. Herein, we employed two antibiotics: ampicillin and ceftriaxone, conjugated with the nanocarrier zinc oxide and β-cyclodextrin, and tested them against via amoebicidal, amoebistatic, encystment, excystment, cytopathogenicity, and cytotoxicity assays at a concentration of 100 μg/mL. Notably, zinc oxide β-cyclodextrin ceftriaxone significantly inhibited growth and cytopathogenicity. Additionally, both zinc oxide β-cyclodextrin ceftriaxone and ceftriaxone markedly inhibited encystment. Furthermore, all the tested compounds displayed negligible cytotoxicity. However, minimal anti-excystment or amoebicidal effects were observed for the compounds. Accordingly, this novel nanoconjugation should be employed in further studies in hope of discovering novel anti- compounds.
是一种无处不在的自由生活变形虫，能够引发人类角膜炎和肉芽肿性阿米巴脑炎。治疗方法仍然有限且不一致。因此，迫切需要新型化合物。纳米技术因增强药物递送和降低毒性而受到关注。先前的研究表明，各种抗生素类别都具有抗阿米巴活性。在此，我们使用了两种抗生素：氨苄青霉素和头孢曲松，它们与纳米载体氧化锌和β-环糊精结合，并在浓度为100μg/mL的情况下通过杀阿米巴、抑制阿米巴生长、包囊形成、脱囊、细胞致病性和细胞毒性试验对其进行测试。值得注意的是，氧化锌β-环糊精头孢曲松显著抑制了生长和细胞致病性。此外，氧化锌β-环糊精头孢曲松和头孢曲松都显著抑制了包囊形成。此外，所有测试化合物的细胞毒性都可忽略不计。然而，观察到这些化合物的脱囊或杀阿米巴作用极小。因此，这种新型纳米共轭物应在进一步研究中使用，以期发现新型抗化合物。
（注：原文中部分“ ”指代不明，翻译时保留原样。）
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