Zhang Tengyue, Ding Yuexia, Peng Jinju, Dai Yue, Luo Shuaishuai, Liu Wenchao, Ma Yi
Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Department of Animal Science, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Antibiotics (Basel). 2022 Sep 23;11(10):1299. doi: 10.3390/antibiotics11101299.
This study evaluates the effects of a broad-spectrum antibiotic (florfenicol) on antibiotic resistance genes (ARGs) and bacterial community structure in aquatic environments. We constructed an indoor aquatic microcosm model, adding different concentrations of florfenicol (0.1, 1, 10, 100 mg L), and water and sediment samples were collected after 0, 7, 30, and 60 days. qPCR and 16S rDNA amplicon sequencing were used to study the changes in the ARGs and bacterial community structure of the collected samples. The results show that the inclusion of florfenicol resulted in an increased abundance of the and genes. Adding 100 mg L florfenicol to the water increased the abundance of gene copies with the maximum on the Day 7, and increased the abundance of gene copies with the maximum on Day 30. Adding 100 mg L florfenicol to the sediment increased the abundance of and genes by one order of magnitude on Day 60. Meanwhile, the average number of operational taxonomic units (OTUs) in the water samples was 257, and the average number of OTUs in sediment samples was 823. The bacterial community diversity and richness in sediments were higher than those in water. The difference between the maximal and minimal values of the Shannon diversity index in the water and sediment samples was 4.36 and 1.95, respectively. The effect of florfenicol on the bacterial community structure in water was much higher than that in sediment. At 30 days, the diversity index and richness index of the florfenicol treatment groups with 1 and 10 mg L concentrations began to increase; at 60 days, the diversity and richness indices of the 100 mg L florfenicol treatment group began to increase. The samples at the same sampling time in the sediments clustered closer together. The results of this study provide a scientific basis for guiding the rational use of florfenicol in aquaculture, maintaining a healthy and stable microecological environment in aquaculture, and provide theoretical data for environmental ecological risk assessment and safety management caused by microbial resistance under the abuse of florfenicol.
本研究评估了一种广谱抗生素（氟苯尼考）对水生环境中抗生素抗性基因（ARGs）和细菌群落结构的影响。我们构建了一个室内水生微宇宙模型，添加不同浓度的氟苯尼考（0.1、1、10、100毫克/升），并在0、7、30和60天后采集水和沉积物样本。采用qPCR和16S rDNA扩增子测序研究采集样本中ARGs和细菌群落结构的变化。结果表明，添加氟苯尼考导致了 和 基因丰度的增加。向水中添加100毫克/升氟苯尼考增加了 基因拷贝数丰度，在第7天达到最大值，增加了 基因拷贝数丰度，在第30天达到最大值。向沉积物中添加100毫克/升氟苯尼考在第60天使 和 基因丰度增加了一个数量级。同时，水样中的平均可操作分类单元（OTU）数量为257，沉积物样本中的平均OTU数量为823。沉积物中的细菌群落多样性和丰富度高于水中。水和沉积物样本中香农多样性指数的最大值与最小值之差分别为4.36和1.95。氟苯尼考对水中细菌群落结构的影响远高于沉积物。在30天时，浓度为1和10毫克/升的氟苯尼考处理组的多样性指数和丰富度指数开始增加；在60天时，100毫克/升氟苯尼考处理组的多样性和丰富度指数开始增加。沉积物中同一采样时间的样本聚类更紧密。本研究结果为指导氟苯尼考在水产养殖中的合理使用、维持水产养殖中健康稳定的微生态环境提供了科学依据，并为氟苯尼考滥用导致的微生物抗性引起的环境生态风险评估和安全管理提供了理论数据。
