da Silva Ana Raquel Pereira, Costa Maria do Socorro, Araújo Nara Juliana Santos, de Freitas Thiago Sampaio, de Almeida Ray Silva, Barbosa Filho José Maria, Tavares Josean Fechine, de Souza Erlânio Oliveira, de Farias Pablo Antonio Maia, Pinheiro Jacqueline Cosmo Andrade, Coutinho Henrique Douglas Melo
Graduate Program in Biotechnology, Universidade Estadual do Ceará-UECE, Fortaleza 60714-903, Brazil.
Graduate Program in Health Sciences, Universidade Federal do Cariri-UFCA, Barbalha 63180-000, Brazil.
Antibiotics (Basel). 2022 Sep 27;11(10):1309. doi: 10.3390/antibiotics11101309.
A worrisome fact is the increase in microbial resistance, which has as its main cause the indiscriminate use of antibiotics. Scientific studies have investigated bioactive compounds such as steroidal sapogenins, in the perspective of new beneficial alternatives for the control of bacterial resistance. Therefore, the objective of this work was to verify the antibacterial activity as well as the modifying action of antibiotics associated with solasodine and its ability to inhibit the efflux pump mechanism in strains of . Tests were performed to verify the minimum inhibitory concentration (MIC). In addition, the action-modifying potential of antibiotics and the inhibitory capacity of the efflux pump NorA and MepA through synergistic effects on the antibiotic and ethidium bromide were evaluated. Solasodine showed significant results for the standard bacteria with an MIC of 512 μg/mL, and when associated with the antibiotics gentamicin and nofloxacin for the multidrug-resistant bacteria 10, 06, and 24, it showed a 50% reduction in MIC. The association of solasodine with the antibiotic ciprofloxacin against K2068 (MepA) showed synergism, with a reduction in the MIC of the antibiotic from 64 μg/mL to 40 μg/mL, and also a reduction in the MIC when the antibiotic was used in conjunction with the efflux pump inhibitors. Solasodine may be acting on the mechanism of action of the antibiotic, as it has shown a potentiating effect when associated with antibiotics, inducing a reduction in the MIC against Gram-positive and Gram-negative bacteria. Therefore, this study demonstrated significant results for the potentiating action of solasodine when associated with antibiotics of clinical importance.
一个令人担忧的事实是微生物耐药性的增加，其主要原因是抗生素的滥用。科学研究已经从控制细菌耐药性的新的有益替代方案的角度，对甾体皂苷元等生物活性化合物进行了研究。因此，本研究的目的是验证与茄解碱相关的抗菌活性、抗生素的修饰作用及其抑制菌株外排泵机制的能力。进行了测试以验证最低抑菌浓度（MIC）。此外，还评估了抗生素的作用修饰潜力以及外排泵NorA和MepA通过对抗生素和溴化乙锭的协同作用的抑制能力。茄解碱对标准细菌显示出显著结果，MIC为512μg/mL，当与庆大霉素和诺氟沙星联合用于多重耐药细菌10、06和24时，MIC降低了50%。茄解碱与抗生素环丙沙星联合对K2068（MepA）显示出协同作用，抗生素的MIC从64μg/mL降至40μg/mL，并且当抗生素与外排泵抑制剂联合使用时MIC也降低。茄解碱可能作用于抗生素的作用机制，因为它与抗生素联合使用时显示出增强作用，导致对革兰氏阳性和革兰氏阴性细菌的MIC降低。因此，本研究证明了茄解碱与具有临床重要性的抗生素联合使用时增强作用的显著结果。
