Zhuang Yu-Wen, Wu Cun-En, Zhou Jin-Yong, Chen Xu, Wu Jian, Jiang Shan, Peng Hai-Yan, Zou Xi, Liu Jia-Yun, Wu Da-Peng, Gong Tao, Qi Ming-Hao, Xue Tian, Liu Shen-Lin, Cai Hui
Department of Integrated Traditional and Western Medicine, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, China.
Cancer Sci. 2017 Nov;108(11):2248-2264. doi: 10.1111/cas.13354. Epub 2017 Sep 14.
Solasodine is a main active component isolated from Solanum incanum L. that performs a wide range of functions containing anti-oxidant, anti-infection, and neurogenesis promotion. In this study, we explored the influence of solasodine on three types of human colorectal cancer (CRC) cell lines. The results show that solasodine prohibited CRC cell proliferation dose- and time-dependently and impeded CRC cell motility by downregulating MMPs. Solasodine was also found to fuel caspase-cascade reaction and increase the ratio between Bax and Bcl-2 so as to induce CRC cell apoptosis. When cells were pretreated with AKT activator (insulin-like growth factor-1) followed by solasodine, the solasodine-induced apoptosis was partially abrogated by insulin-like growth factor-1. Moreover, solasodine hindered tumor development and stimulated similar mechanisms in vivo. In general, our study provides the first evidence that solasodine has a suppressive effect on CRC cells and that this agent may be a novel therapeutic drug for CRC treatment.
澳洲茄碱是从白英中分离出的一种主要活性成分，具有多种功能，包括抗氧化、抗感染和促进神经发生。在本研究中，我们探讨了澳洲茄碱对三种人类结肠癌细胞系的影响。结果表明，澳洲茄碱剂量和时间依赖性地抑制结肠癌细胞增殖，并通过下调基质金属蛋白酶来阻碍结肠癌细胞的运动。还发现澳洲茄碱可促进半胱天冬酶级联反应，并增加Bax与Bcl-2的比例，从而诱导结肠癌细胞凋亡。当细胞先用AKT激活剂（胰岛素样生长因子-1）预处理，然后再用澳洲茄碱处理时，胰岛素样生长因子-1可部分消除澳洲茄碱诱导的凋亡。此外，澳洲茄碱在体内可阻碍肿瘤发展并激发类似机制。总的来说，我们的研究首次证明澳洲茄碱对结肠癌细胞具有抑制作用，并且该药物可能是一种用于治疗结肠癌的新型治疗药物。
