Ribeiro Adriana Barbosa, Ribeiro Aline Barbosa, de Araújo Camila Borba, Fortes Caroline Vieira, Clemente Lorena Mosconi, Paranhos Helena de Freitas Oliveira, Watanabe Evandro, Salgado Helio Cesar, Silva-Lovato Cláudia Helena
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, Brazil.
Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14040-904, Brazil.
Antibiotics (Basel). 2022 Sep 28;11(10):1320. doi: 10.3390/antibiotics11101320.
Denture-related stomatitis (DRS) is frequent oral inflammation in complete denture wearers. This study evaluated the effect of a hygiene protocol on DRS remission, local inflammatory factors, and hemodynamic responses. Thirty-three individuals were enrolled in the study. The outcomes were measured before and after 10 days of a hygiene protocol treatment consisting of brushing the palate with a soft brush and water and denture brushing with a denture-specific brush and mild soap, as well as immersion of the denture for 20 min in a 0.25% sodium hypochlorite solution. Data were analyzed by paired Wilcoxon for biofilm removal and CFU count of microorganisms. The paired T test was used to assess salivary MUC 1, cytokines, and arterial pressure (p < 0.05). A significant difference was found in the DRS degree (p < 0.001), biofilm (p < 0.001), microbial load of Candida spp. (p < 0.001), Gram-negative (p < 0.004), Staphylococcus spp. (p < 0.001), and S. mutans (p < 0.001) of the denture, and S. mutans (p < 0.001) of the palate after use of the protocol. The salivary flow (p = 0.2) and pH (p = 0.97) did not change; there was an increase of MUC 1 (p = 0.049) and a decrease in IL-6 (p = 0.038), IL-2 (p = 0.04), IL-10 (p = 0.041), and IFNγ (p = 0.04). There was also a decrease in systolic (p = 0.012) and mean arterial pressure (p = 0.02). The current hygiene protocol reduced the inflammation degree of DRS and promoted an improvement of local inflammatory factors and a reduction in the systolic arterial pressure of the patients.
义齿性口炎（DRS）是全口义齿佩戴者常见的口腔炎症。本研究评估了一种卫生方案对DRS缓解、局部炎症因子和血流动力学反应的影响。33名个体参与了本研究。在一项卫生方案治疗10天前后测量结果，该方案包括用软刷和水刷洗腭部、用义齿专用刷和温和肥皂刷洗义齿，以及将义齿浸泡在0.25%次氯酸钠溶液中20分钟。采用配对Wilcoxon检验分析生物膜清除和微生物的菌落形成单位计数数据。采用配对t检验评估唾液MUC 1、细胞因子和动脉压（p<0.05）。发现义齿的DRS程度（p<0.001）、生物膜（p<0.001）、念珠菌属微生物载量（p<0.001）、革兰氏阴性菌（p<0.004）、葡萄球菌属（p<0.001）和变形链球菌（p<0.001）以及使用该方案后腭部的变形链球菌（p<0.001）有显著差异。唾液流量（p = 0.2）和pH值（p = 0.97）没有变化；MUC 1增加（p = 0.049），IL-6（p = 0.038）、IL-2（p = 0.04）、IL-10（p = 0.041）和IFNγ（p = 0.04）减少。收缩压（p = 0.012）和平均动脉压也降低（p = 0.02）。当前的卫生方案降低了DRS的炎症程度，促进了局部炎症因子的改善，并降低了患者的收缩压。
