Wang Ping, Wang Hongou, Liu Cunwei, Feng Chengjie, Lu Qinghui, Zou Qinghua
Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Peking University People's Hospital, Beijing 100044, China.
Antibiotics (Basel). 2022 Oct 9;11(10):1379. doi: 10.3390/antibiotics11101379.
is one of the main pathogens causing nosocomial and community-acquired infections. Tigecycline is an important antibiotic for the treatment of multidrug-resistant infections, but strains resistant to tigecycline have also emerged. There are still many unclear questions concerning the mechanism of tigecycline resistance in . In this study, tigecycline-susceptible and tigecycline-intermediate strains were gradually cultured with sub-minimum inhibitory concentrations of tigecycline to select for tigecycline-resistant mutants, and a tigecycline-resistant strain was cultured under 42 °C to select for tigecycline-susceptible mutants. We found that the acquisition of tigecycline resistance affected the susceptibility of the strains to other antibiotics. Resistance to ampicillin-sulbactam is negatively correlated with tigecycline resistance. The strains will experience fitness costs along with the acquisition of tigecycline resistance. Tigecycline resistance in the strains was not related to 16S rRNA target variation or outer membrane integrity alteration. By constructing a transposon mutation library, we found that transposon insertion of the gene reduced the sensitivity of to tigecycline. This study provides important clues for understanding the mechanism of tigecycline resistance in .
是引起医院感染和社区获得性感染的主要病原体之一。替加环素是治疗多重耐药感染的重要抗生素，但对替加环素耐药的菌株也已出现。关于……中替加环素耐药机制仍有许多不清楚的问题。在本研究中，用低于最低抑菌浓度的替加环素逐步培养替加环素敏感菌株和替加环素中介菌株以筛选出替加环素耐药突变体，并且在42℃下培养一株替加环素耐药菌株以筛选出替加环素敏感突变体。我们发现获得替加环素耐药性会影响菌株对其他抗生素的敏感性。对氨苄西林-舒巴坦的耐药性与替加环素耐药性呈负相关。菌株在获得替加环素耐药性的同时会经历适应性代价。菌株中的替加环素耐药性与16S rRNA靶标变异或外膜完整性改变无关。通过构建转座子突变文库，我们发现……基因的转座子插入降低了……对替加环素的敏感性。本研究为理解……中替加环素耐药机制提供了重要线索。
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