早期妊娠江淮水牛环状RNA的表达谱分析
Expression Profiling of Circular RNAs in Early Pregnant Jianghuai Buffaloes.
作者信息
Liu Qiuchen, Zhang Mengya, Guo Tenglong, Wu Sucheng, Zong Yanfeng, Xu Changzhi, Zhu Zhihua, Zhang Yunhai, Cao Zubing
机构信息
Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Department of Animal Engineering, Huaian Bioengineering Vocational College, Huaian 223001, China.
出版信息
Animals (Basel). 2022 Oct 13;12(20):2748. doi: 10.3390/ani12202748.
Circular RNA (circRNA) is expressed in cells and tissues of several species. However, the expression of circRNAs in the blood of Jianghuai buffaloes during early pregnancy has not been reported. In this study, we identified the DECs in the blood of Jianghuai buffaloes and annotated the functions of these DECs. The results showed that there were 890 DECs between the pregnant and non-pregnant groups, of which more than 80% were exon-derived circRNAs, including 323 up-regulated circRNAs and 567 down-regulated circRNAs. Enrichment analysis revealed that DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway important for embryonic development and pregnancy maintenance. In addition, most DECs have multiple miRNA targets, suggesting that these DECs have the potential to function as miRNA sponges. In conclusion, several DECs are present between pregnant and non-pregnant Jianghuai buffaloes, and these DECs are associated with embryo implantation and pregnancy establishment.
环状RNA（circRNA）在多个物种的细胞和组织中均有表达。然而，江淮水牛妊娠早期血液中circRNAs的表达情况尚未见报道。在本研究中，我们鉴定了江淮水牛血液中的差异表达环状RNA（DECs）并对其功能进行了注释。结果显示，妊娠组和未妊娠组之间共有890个DECs，其中80%以上为外显子来源的circRNAs，包括323个上调的circRNAs和567个下调的circRNAs。富集分析表明，DECs主要富集于对胚胎发育和维持妊娠至关重要的表皮生长因子受体信号通路。此外，大多数DECs具有多个微小RNA（miRNA）靶点，提示这些DECs具有作为miRNA海绵发挥作用的潜力。总之，妊娠和未妊娠的江淮水牛之间存在多个DECs，这些DECs与胚胎着床和妊娠建立相关。
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