Roman Katarzyna, Wilk Martyna, Książek Piotr, Czyż Katarzyna, Roman Adam
Department of Animal Nutrition and Feed Science, Wroclaw University of Environmental and Life Sciences, 51-630 Wroclaw, Poland.
Independent Researcher, 51-649 Wroclaw, Poland.
Animals (Basel). 2022 Oct 14;12(20):2773. doi: 10.3390/ani12202773.
The composition of the diet, including the amount and type of lipid supplements, influences the products formed in the digestive tract, their levels in the blood and their deposition in the tissues. One example of a plant rich in polyunsaturated fatty acids is flax (Linum usitatissimum). The aim of the presented work was to evaluate the effect of the linseed oil ethyl esters additive and environmental conditions on the selected rabbit blood parameters. The study included two types of animal maintenance (laboratory conditions and external conditions) and two seasons (summer and winter), a total of four study stages. At each stage, a control group and an experimental group were specified. The feed supplement in the form of esterified linseed oil was administered to the experimental animals for two months. The blood samples were collected at the beginning of the experiment, as well as at the end of treatment. Moreover, blood samples were collected also after two months from the end of the experiment. During the experiment, morphological and biochemical parameters of the rabbits’ blood were examined. The results of the content of fatty acids were determined in the erythrocytes and serum blood samples and grouped into saturated and unsaturated fatty acids, especially omega-3 and omega-6 fatty acids. In an internal study, the influence of housing conditions and season on selected morphological and biochemical parameters of rabbit blood was confirmed. Furthermore, expected beneficial changes in the fatty acid profile in erythrocytes and blood serum were observed as a result of supplementation. A significant increase in omega-3 fatty acids was noted as well as a substantial decrease in the ratio of omega-6 to omega-3 fatty acids (p < 0.01). Most importantly, the linseed oil ethyl ester supplement used did not adversely affect the health of the rabbits, as evidenced by biochemical and blood morphological indices remaining within, known so far, reference limits or showing only slight fluctuations (p > 0.05). However, the obtained results extend the database of hematological blood parameters in rabbits, thus, contributing to improvements in laboratory diagnostics for fur animals.
饮食的组成，包括脂质补充剂的数量和类型，会影响消化道中形成的产物、它们在血液中的水平以及在组织中的沉积。富含多不饱和脂肪酸的一种植物示例是亚麻（亚麻籽）。本研究的目的是评估亚麻籽油乙酯添加剂和环境条件对所选家兔血液参数的影响。该研究包括两种动物饲养方式（实验室条件和外部条件）以及两个季节（夏季和冬季），总共四个研究阶段。在每个阶段，都指定了一个对照组和一个实验组。以酯化亚麻籽油形式的饲料补充剂给予实验动物两个月。在实验开始时以及治疗结束时采集血样。此外，在实验结束两个月后也采集血样。在实验过程中，检测了家兔血液的形态学和生化参数。测定了红细胞和血清血样中脂肪酸的含量，并将其分为饱和脂肪酸和不饱和脂肪酸，特别是ω-3和ω-6脂肪酸。在一项内部研究中，证实了饲养条件和季节对家兔血液所选形态学和生化参数的影响。此外，由于补充，观察到红细胞和血清中脂肪酸谱出现了预期的有益变化。ω-3脂肪酸显著增加，ω-6与ω-3脂肪酸的比例大幅下降（p < 0.01）。最重要的是，所用的亚麻籽油乙酯补充剂并未对家兔健康产生不利影响，生化和血液形态学指标保持在目前已知的参考范围内或仅显示轻微波动（p > 0.05）即可证明。然而，所获得的结果扩展了家兔血液血液学参数的数据库，从而有助于改进毛皮动物的实验室诊断。
