Bellatreche Aicha Yasmine, Bouzid Riad, Blaizot Amandine, Aubert Dominique, Blaga Radu, Ait-Oudhia Khatima, Le Roux Delphine
Ecole Nationale Supérieure Vétérinaire, Rue Issad Abbes, Oued Smar, El Harrach, Algiers 16000, Algeria.
Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, Laboratoire de Santé Animale, BIPAR, F-94700 Maisons-Alfort, France.
Animals (Basel). 2022 Oct 18;12(20):2813. doi: 10.3390/ani12202813.
Toxoplasmosis is a zoonotic disease, caused by the protozoan , affecting most warm-blooded animals. Assessing the seroprevalence of in different animal species gives a good estimate of the global circulation of the parasite and the risk for human infections. However, the seroprevalence of in dogs is not studied as much as other species, despite their close contact with wildlife and humans in rural or urban environments and evidence that dogs can also be a potential source for human contaminations. A commercial enzyme-inked immunosorbent assay (ELISA) kit to detect anti- antibodies in sera of hunting dogs potentially naturally infected, was compared to the modified agglutination test (MAT), used as the reference method. The ELISA presented a sensitivity of 76.5% (CI 95%: 60.0-87.6) and a specificity of 87.7% (CI 95%: 76.7-93.9) and a substantial agreement with the MAT for the detection of canine anti- antibodies. Both tests can therefore be used widely for epidemiology studies on infections in dogs. With a mean seroprevalence of infection in hunting dogs from northern Algeria of 36.8% (CI 95%: 34.9-38.7), this study also highlights the importance of seroprevalence studies in companion animals to assess infectious risk for human populations.
弓形虫病是一种人畜共患疾病，由原生动物引起，可感染大多数温血动物。评估不同动物物种中的血清阳性率有助于很好地估计该寄生虫在全球的传播情况以及人类感染风险。然而，尽管狗在农村或城市环境中与野生动物和人类密切接触，且有证据表明狗也可能是人类感染的潜在来源，但对狗的血清阳性率的研究不如对其他物种那么多。将一种用于检测可能自然感染的猎犬血清中抗[具体抗体名称]抗体的商业酶联免疫吸附测定（ELISA）试剂盒与用作参考方法的改良凝集试验（MAT）进行了比较。ELISA的灵敏度为76.5%（95%置信区间：60.0 - 87.6），特异性为87.7%（95%置信区间：76.7 - 93.9），并且在检测犬抗[具体抗体名称]抗体方面与MAT有高度一致性。因此，这两种检测方法均可广泛用于狗弓形虫感染的流行病学研究。阿尔及利亚北部猎犬的弓形虫感染平均血清阳性率为36.8%（95%置信区间：34.9 - 38.7），该研究还强调了对伴侣动物进行弓形虫血清阳性率研究以评估人群感染风险的重要性。
