de Almeida Jonatas Campos, Frehse Michelle Salmon, Navarro Italmar Teodorico, Garcia João Luis, Biondo Alexander Welker, Freire Roberta Lemos
Acta Parasitol. 2016 Dec 1;61(4):694-696. doi: 10.1515/ap-2016-0097.
The aim of the present study was to determine the prevalence of antibodies to Toxoplasma gondii by two serological techniques in sera of 364 stray dogs from Brazil by immunofluorescence antibody test (IFAT, cut off point 1:16) and to the modified agglutination test (MAT, cut-off points 1:25 and 1:50). A total of 175/364 (48.07%) sera were positive by IFAT, and 108/364 (29.67%) and 85/364 (23.35%) were positive by MAT with cutoff points 1:25 and 1:50, respectively were positive by MAT. Cohen's Kappa Coefficient between IFAT and MAT was 0.81 (excellent) and 0.66 (substantial) with cutoff points 1:25 and 1:50, respectively. Using IFAT as gold standard, MAT sensitivity and specificity were 78% and 99% for 1:25 and 61% and 99% for 1:50, respectively. The results document of the usefulness of MAT for serological diagnosis because it does not require species-specific conjugate.
本研究的目的是通过免疫荧光抗体试验（IFAT，截断值为1:16）和改良凝集试验（MAT，截断值为1:25和1:50）这两种血清学技术，测定来自巴西的364只流浪狗血清中抗弓形虫抗体的流行情况。通过IFAT检测，共有175/364（48.07%）的血清呈阳性；通过MAT检测，截断值为1:25时，108/364（29.67%）的血清呈阳性，截断值为1:50时，85/364（23.35%）的血清呈阳性。IFAT与MAT之间的Cohen's Kappa系数在截断值为1:25和1:50时分别为0.81（优秀）和0.66（高度一致）。以IFAT作为金标准，MAT在截断值为1:25时的敏感性和特异性分别为78%和99%，在截断值为1:50时分别为61%和99%。结果证明了MAT用于血清学诊断的有效性，因为它不需要种属特异性结合物。
