Ferreira Jorge M, Jorge Sara, Félix Luís, Morello Gabriela M, Olsson I Anna S, Valentim Ana M
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.
IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal.
Biology (Basel). 2022 Sep 30;11(10):1433. doi: 10.3390/biology11101433.
The use of zebrafish () as an animal model is growing and occurs in a wide range of scientific areas. Therefore, researchers need better and more appropriate anaesthetics for stressful and/or painful procedures to prevent unpleasant experiences. Thus, we aimed to study if adult zebrafish displayed aversion-associated behaviours (conditioned place aversion) and alterations in cortisol levels when exposed to equipotent concentrations of MS222, propofol/lidocaine, clove oil, or etomidate. Adult AB zebrafish (mixed-sex, N = 177) were randomly assigned to MS222 (150 mg/L), Propofol/Lidocaine (5 mg/L propofol + 150 mg/L lidocaine), Clove Oil (45 mg/L), or Etomidate (2 mg/L) groups. The conditioned place aversion test was used to assess behavioural aversion. Only etomidate resulted in a similar aversion to the positive control group (HCl; pH = 3). Cortisol levels were measured 5 and 15 min after loss of equilibrium. Etomidate induced low levels of cortisol by impairing its synthesis, whereas all the other groups had similar cortisol levels. Based on our data, etomidate was ruled out as an alternative to MS222, as it showed an aversive profile. The remaining protocols were not innocuous, displaying a weak aversive profile when compared to the positive control. In conclusion, a combination of propofol with lidocaine, clove oil, and MS222 were valid candidates for use as anaesthetic protocols.
斑马鱼作为一种动物模型的应用正在不断增加，并且出现在广泛的科学领域。因此，研究人员需要更好、更合适的麻醉剂来应对压力和/或痛苦的操作，以避免不愉快的经历。因此，我们旨在研究成年斑马鱼在暴露于等效浓度的MS222、丙泊酚/利多卡因、丁香油或依托咪酯时，是否表现出厌恶相关行为（条件性位置厌恶）以及皮质醇水平的变化。成年AB品系斑马鱼（雌雄混合，N = 177）被随机分配到MS222（150 mg/L）、丙泊酚/利多卡因（5 mg/L丙泊酚 + 150 mg/L利多卡因）、丁香油（45 mg/L）或依托咪酯（2 mg/L）组。采用条件性位置厌恶试验来评估行为厌恶。只有依托咪酯导致与阳性对照组（盐酸；pH = 3）相似的厌恶反应。在失去平衡后5分钟和15分钟测量皮质醇水平。依托咪酯通过损害其合成诱导低水平的皮质醇，而所有其他组的皮质醇水平相似。根据我们的数据，依托咪酯因表现出厌恶特征而被排除作为MS222的替代品。其余方案并非无害，与阳性对照相比显示出较弱的厌恶特征。总之，丙泊酚与利多卡因、丁香油和MS222的组合是用作麻醉方案的有效候选者。
