Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Lianshui Peoples Hospital, Huaian 223400, China.
Biosensors (Basel). 2022 Sep 27;12(10):797. doi: 10.3390/bios12100797.
The timelier and more accurate the diagnosis of the disease, the higher the patient's survival rate. Human epididymal protein 4 (HE4) has great significance as a biomarker of concern for reflecting ovarian cancer. Herein, we prepared a novel optical label that can be used in lateral-flow immuno-dipstick assay (LFIA) for sensitive visual detection of HE4 by implanting hydrophobic gold nanoparticles (Au NPs) at high density in Mg/Fe LDH nanoflowers (MF NFs). MF NFs with large specific surface area, high porosity, abundant active binding sites, and stable structure were employed for the first time as templates to directly anchor Au NPs in the organic phase. After simple modification with an optimized amount of branched polyethyleneimine, not only could MF@Au NFs be dispersed in the aqueous phase, but also amino functional groups were introduced on its surface to facilitate subsequent antibody coupling steps. The limit of detection reaches 50 pM with a detection range of 50 to 1000 pM. This work initially explored how MF NFs can be used to load signal labels with ideal stability and signal amplification capabilities, which greatly improves the practicability of LFIA and highlights its important role in the field of rapid diagnostics.
疾病诊断得越早、越准确，患者的生存率就越高。人附睾蛋白 4（HE4）作为一种反映卵巢癌的生物标志物，具有重要意义。在此，我们制备了一种新型光学标记物，可用于侧向流动免疫层析测定法（LFIA），通过在 Mg/Fe 层状双氢氧化物纳米花（MF NFs）中高密度植入疏水性金纳米颗粒（Au NPs），对 HE4 进行灵敏的可视化检测。MF NFs 具有大的比表面积、高的孔隙率、丰富的活性结合位点和稳定的结构，首次被用作模板，将 Au NPs 直接固定在有机相中。经过优化量的支化聚乙烯亚胺的简单修饰后，不仅 MF@Au NFs 可以分散在水相中，而且在其表面引入了氨基官能团，有利于后续的抗体偶联步骤。检测限达到 50 pM，检测范围为 50 到 1000 pM。这项工作初步探索了 MF NFs 如何用于负载具有理想稳定性和信号放大能力的信号标记物，极大地提高了 LFIA 的实用性，并突出了其在快速诊断领域的重要作用。
