Hlisníková Henrieta, Nagyová Miroslava, Kolena Branislav, Mlynček Miloš, Trnovec Tomáš, Petrovičová Ida
Department of Zoology and Anthropology, Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra, 94974 Nitra-Chrenová, Slovakia.
Department of Nursing, Faculty of Social Sciences and Health Care, Constantine the Philosopher University in Nitra, 94974 Nitra-Chrenová, Slovakia.
Children (Basel). 2022 Oct 15;9(10):1561. doi: 10.3390/children9101561.
Phthalates alter the hormonal balance in humans during pregnancy, potentially affecting embryonic and fetal development. We studied the joint effect of exposure to phthalates, quantified by urinary phthalate metabolite concentration, and perceived psychological stress on the concentration of hormones in pregnant women ( = 90) from the Nitra region, Slovakia, up to the 15th week of pregnancy. We used high-performance liquid chromatography, tandem mass spectrometry (HPLC-MS/MS), and electro-chemiluminescence immunoassay to determine urinary concentrations of phthalates and serum concentrations of hormones, respectively. We used Cohen perceived stress scale (PSS) to evaluate the human perception of stressful situations. Our results showed that mono(carboxy-methyl-heptyl) phthalate (cx-MiNP) and a molar sum of di-iso-nonyl phthalate metabolites (ΣDiNP) were negatively associated with luteinizing hormone (LH) ( ≤ 0.05). Mono(hydroxy-methyl-octyl) phthalate (OH-MiNP) and the molar sum of high-molecular-weight phthalate metabolites (ΣHMWP) were positively associated with estradiol ( ≤ 0.05). PSS score was not significantly associated with hormonal concentrations. When the interaction effects of PSS score and monoethyl phthalate (MEP), cx-MiNP, ΣDiNP, and ΣHMWP on LH were analyzed, the associations were positive ( ≤ 0.05). Our cross-sectional study highlights that joint psychosocial stress and xenobiotic-induced stress caused by phthalates are associated with modulated concentrations of reproductive hormones in pregnant women.
邻苯二甲酸盐会改变人类孕期的激素平衡，可能影响胚胎和胎儿发育。我们研究了斯洛伐克尼特拉地区90名孕妇在怀孕15周前，通过尿中邻苯二甲酸酯代谢物浓度量化的邻苯二甲酸盐暴露与感知到的心理压力对激素浓度的联合影响。我们分别使用高效液相色谱-串联质谱法（HPLC-MS/MS）和电化学发光免疫分析法来测定尿中邻苯二甲酸盐浓度和血清激素浓度。我们使用科恩感知压力量表（PSS）来评估人类对压力状况的感知。我们的结果表明，单（羧基甲基庚基）邻苯二甲酸酯（cx-MiNP）和邻苯二甲酸二异壬酯代谢物的摩尔总和（ΣDiNP）与促黄体生成素（LH）呈负相关（P≤0.05）。单（羟甲基辛基）邻苯二甲酸酯（OH-MiNP）和高分子量邻苯二甲酸酯代谢物的摩尔总和（ΣHMWP）与雌二醇呈正相关（P≤0.05）。PSS评分与激素浓度无显著相关性。当分析PSS评分与邻苯二甲酸单乙酯（MEP）、cx-MiNP、ΣDiNP和ΣHMWP对LH的交互作用时，相关性为正（P≤0.05）。我们的横断面研究强调，心理社会压力和邻苯二甲酸盐引起的外源性应激与孕妇生殖激素浓度的调节有关。
