Takano K I, Sugiyama S, Nakazawa S, Ozawa T
Scand J Gastroenterol. 1987 Jun;22(5):577-84. doi: 10.3109/00365528708991901.
This study was designed to establish the role of tissue phospholipase (PLase) and prostaglandins (PGs) in the genesis of gastric ulcers. The activity of PLase and levels of PGs in rat gastric mucosa were measured by high-performance liquid chromatography, using water immersion stress ulcer. Four kinds of PGs in gastric mucosa were determined: 6-keto-PGF1 alpha, PGF2 alpha, PGE2, and PGD2. Decreases in PG levels and in PLase activity were observed, depending on the duration of water immersion stress. Either administration of pirenzepine or vagotomy maintained both the PG levels and PLase activity. The longer duration of stress induced a more severe ulcer index. Premedication with pirenzepine or vagotomy prevented ulceration. Furthermore, there was a clear reciprocal correlation between the PLase activity and the ulcer index. The decrease in PG levels observed could be linked with the decrease in the activity of PLase, the initiating enzyme of the arachidonate cascade reaction. Thus, PLase might be a responsible factor in the genesis of stress ulcers. Pirenzepine or vagotomy protected gastric mucosa from ulceration by maintaining the PLase activity and PG levels.
本研究旨在确定组织磷脂酶（PLase）和前列腺素（PGs）在胃溃疡发生过程中的作用。使用水浸应激性溃疡模型，通过高效液相色谱法测定大鼠胃黏膜中PLase的活性和PGs的水平。测定胃黏膜中的四种PGs：6-酮-前列腺素F1α、前列腺素F2α、前列腺素E2和前列腺素D2。观察到PGs水平和PLase活性随水浸应激时间的延长而降低。给予哌仑西平或进行迷走神经切断术均可维持PGs水平和PLase活性。应激时间越长，溃疡指数越严重。术前给予哌仑西平或进行迷走神经切断术可预防溃疡形成。此外，PLase活性与溃疡指数之间存在明显的负相关。观察到的PGs水平降低可能与花生四烯酸级联反应的起始酶PLase活性降低有关。因此，PLase可能是应激性溃疡发生的一个相关因素。哌仑西平或迷走神经切断术通过维持PLase活性和PGs水平来保护胃黏膜免受溃疡形成。
