Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.
Genes (Basel). 2022 Sep 21;13(10):1691. doi: 10.3390/genes13101691.
The complex is a group of closely related desert and steppe subshrubs that includes , and , which are the only sect. components found in Northwest China. Based on earlier molecular evidence, the species boundaries and phylogenetic relationships within this complex remain poorly resolved. Here, we characterized seven complete chloroplast genomes encompassing the representatives of the three taxa in the complex and one closely related species, , as well as three other species of . Comparative genomic analyses indicated that the genomic structure, gene order and content were highly conserved among these eleven plastomes. Highly variable plastid regions and simple sequence repeats (SSRs) were identified. The robust and consistent phylogenetic relationships of the complex were firstly constructed based on a total of 26 plastid genomes from Scrophulariaceae. Within the monophyletic complex, a individual from Pamirs Plateau was identified as the earliest diverging clade, followed by from Tibet, while the remaining individuals of from the Tianshan Mountains and from Qinghai-Gansu were clustered into sister clades. Our results evidently demonstrate the capability of plastid genomes to improve phylogenetic resolution and species delimitation, particularly among closely related species, and will promote the understanding of plastome evolution in .
该复合体是一组密切相关的沙漠和草原亚灌木，包括 、 和 ，它们是中国西北地区仅有的 sect. 成分。基于早期的分子证据，该复合体中种间的界限和系统发育关系仍未得到很好的解决。在这里，我们对该复合体的三个代表种和一个近缘种 ，以及 属的三个其他种的七个完整叶绿体基因组进行了特征描述。比较基因组分析表明，这 11 个叶绿体基因组在基因组结构、基因顺序和内容上高度保守。鉴定出高度可变的质体区和简单序列重复 (SSR)。基于来自玄参科的 26 个质体基因组，首次构建了 复合体的稳健而一致的系统发育关系。在单系复合体中，来自帕米尔高原的 个体被确定为最早分化的分支，其次是来自西藏的 ，而天山山脉的其余 和来自青海-甘肃的 则聚类为姐妹分支。我们的研究结果清楚地表明，质体基因组在提高系统发育分辨率和物种界限方面具有能力，特别是在密切相关的物种中，这将促进对 属质体进化的理解。
