来自喀麦隆北部的对滴滴涕的抗性与表皮烃类的高产量有关。

DDT Resistance in from Northern Cameroon Associated with High Cuticular Hydrocarbon Production.

机构信息

Vector-Borne Diseases Laboratory of the Applied Biology and Ecology Research Unit (VBID-URBEA), Department of Animal Biology, Faculty of Science, University of Dschang, Dschang P.O. Box 067, Cameroon.

Laboratoire de Recherche sur le Paludisme, Organisation de Coordination Pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Yaoundé P.O. Box 288, Cameroon.

出版信息

Genes (Basel). 2022 Sep 25;13(10):1723. doi: 10.3390/genes13101723.

DOI:10.3390/genes13101723

PMID:36292608

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9601446/

Abstract

Despite the contribution of secondary vectors to malaria transmission, there is still not enough information on their susceptibility status to insecticides. The present study assesses the resistance profile of to DDT. WHO tube tests were used to screen mosquito populations collected from the far-north region of Cameroon for susceptibility to 4% DDT. High DDT resistance in populations from Maga, Simatou and Yangah with mortality rates ranging from 62.79% to 80% was recorded. Direct sequencing (Sanger) of the VGSC gene was undertaken to search for L1014F/S mutations. However, no allele was detected in the resistant samples. We then looked for cuticle alterations and CHC identification and quantitation were undertaken using GC-MS and GC-FID. High production of cuticular hydrocarbon was recorded in the populations of Yangah and Simatou, with 2420.9 ± 265 and 2372.5 ± 225 ng CHCs/mg dry weight, respectively. The present findings are the first ever describing the development of cuticle resistance in . The data suggest the need to expand surveillance activities on other vector species.

摘要

尽管次要传播媒介对疟疾传播有贡献，但关于它们对杀虫剂的敏感性状况的信息仍然不足。本研究评估了对滴滴涕的抗药性。使用世界卫生组织管测试法筛选了来自喀麦隆极北地区的蚊子种群，以确定对 4%滴滴涕的敏感性。来自 Maga、Simatou 和 Yangah 的种群对滴滴涕的高度抗药性，死亡率范围为 62.79%至 80%。进行了 VGSC 基因的直接测序（Sanger），以寻找 L1014F/S 突变。然而，在抗性样本中未检测到等位基因。然后，我们寻找表皮改变，并使用 GC-MS 和 GC-FID 进行 CHC 鉴定和定量。Yangah 和 Simatou 种群的表皮碳氢化合物产量很高，分别为 2420.9±265 和 2372.5±225ng CHCs/mg 干重。目前的发现是首次描述表皮抗药性的发展。数据表明需要扩大对其他媒介物种的监测活动。