Ntonga Akono P, Mbouangoro A, Mbida Mbida A, Ndo C, Peka Nsangou M F, Kekeunou S
Laboratoire de biologie et physiologie animale, Département de biologie animale, Faculté des sciences, Université de Douala, BP. 24 157, Douala, Cameroun.
Laboratoire d'écologie et de parasitologie, Département de biologie et physiologie animale, Faculté des sciences, Université de Yaounde I, BP. 812, Yaoundé, Cameroun.
Bull Soc Pathol Exot. 2017 May;110(2):122-129. doi: 10.1007/s13149-017-0553-2. Epub 2017 Mar 16.
This study was conducted from May to June 2015 in Yassa (industrialized area) and Logbessou (non-industrialized area), two peri-urban areas of the city of Douala, Cameroon with the aim of an assessment of the spatial distribution of the gambiae complex, the determination of their resistance to insecticides and the distribution of the Kdr mutation. Mosquito larvae were collected by the dipping method and nursed to adult stage. The sensitivity of adult Anopheles gambiae s.l. populations to DDT and pyrethroids was assessed following WHO protocol. All tested mosquitoes were identified by PCR SINE. Only survivors were used to search for the Kdr mutation. In both areas, the gambiae complex included An. coluzzii and An. gambiae sympatric in their breeding sites. However, An. gambiae was predominant in Logbessou (88%) and An. coluzzii in Yassa (68%). Tests with deltamethrin, permethrin and DDT revealed mortality rates below 90% regardless of the area of origin of the mosquitoes. PCR diagnosis of Kdr mutation showed that over 95% of survivors harbored the Kdr gene in both sites, with resistant allele frequencies ranging from 0.96 to 1.0 in An. gambiae and from 0.89 to 0.96 in An. coluzzii. The strong resistance of An. coluzzii and This study was conducted from May to June 2015 in Yassa (industrialized area) and Logbessou (nonindustrialized area), two peri-urban areas of the city of Douala, Cameroon with the aim of an assessment of the spatial distribution of the gambiae complex, the determination of their resistance to insecticides and the distribution of the Kdr mutation. Mosquito larvae were collected by the dipping method and nursed to adult stage. The sensitivity of adult Anopheles gambiae s.l. populations to DDT and pyrethroids was assessed following WHO protocol. All tested mosquitoes were identified by PCR SINE. Only survivors were used to search for the Kdr mutation. In both areas, the gambiae complex included An. coluzzii and An. gambiae sympatric in their breeding sites. However, An. gambiae was predominant in Logbessou (88%) and An. coluzzii in Yassa (68%). Tests with deltamethrin, permethrin and DDT revealed mortality rates below 90% regardless of the area of origin of the mosquitoes. PCR diagnosis of Kdr mutation showed that over 95% of survivors harbored the Kdr gene in both sites, with resistant allele frequencies ranging from 0.96 to 1.0 in An. gambiae and from 0.89 to 0.96 in An. coluzzii. The strong resistance of An. coluzzii and This study was conducted from May to June 2015 in Yassa (industrialized area) and Logbessou (nonindustrialized area), two peri-urban areas of the city of Douala, Cameroon with the aim of an assessment of the spatial distribution of the gambiae complex, the determination of their resistance to insecticides and the distribution of the Kdr mutation. Mosquito larvae were collected by the dipping method and nursed to adult stage. The sensitivity of adult Anopheles gambiae s.l. populations to DDT and pyrethroids was assessed following WHO protocol. All tested mosquitoes were identified by PCR SINE. Only survivors were used to search for the Kdr mutation. In both areas, the gambiae complex included An. coluzzii and An. gambiae sympatric in their breeding sites. However, An. gambiae was predominant in Logbessou (88%) and An. coluzzii in Yassa (68%). Tests with deltamethrin, permethrin and DDT revealed mortality rates below 90% regardless of the area of origin of the mosquitoes. PCR diagnosis of Kdr mutation showed that over 95% of survivors harbored the Kdr gene in both sites, with resistant allele frequencies ranging from 0.96 to 1.0 in An. gambiae and from 0.89 to 0.96 in An. coluzzii. The strong resistance of An. coluzzii and An. gambiae to insecticides highlights the need for developing new mosquito insecticides.
本研究于2015年5月至6月在喀麦隆杜阿拉市的两个城郊地区亚萨（工业化地区）和洛贝苏（非工业化地区）开展，旨在评估冈比亚蚊复合组的空间分布、确定其对杀虫剂的抗性以及Kdr突变的分布情况。采用浸渍法采集蚊虫幼虫并饲养至成虫阶段。按照世界卫生组织的方案评估成年冈比亚按蚊复合种群对滴滴涕和拟除虫菊酯的敏感性。所有受试蚊虫均通过PCR SINE进行鉴定。仅使用存活者来检测Kdr突变。在这两个地区，冈比亚蚊复合组在其繁殖地包括科氏按蚊和冈比亚按蚊，二者同域分布。然而，冈比亚按蚊在洛贝苏占主导地位（88%），而科氏按蚊在亚萨占主导地位（68%）。使用溴氰菊酯、氯菊酯和滴滴涕进行的测试显示，无论蚊虫的来源地如何，死亡率均低于90%。Kdr突变的PCR诊断表明，在两个地点，超过95%的存活者携带Kdr基因，在冈比亚按蚊中抗性等位基因频率范围为0.96至1.0，在科氏按蚊中为0.89至0.96。科氏按蚊和冈比亚按蚊对杀虫剂的强抗性凸显了开发新型蚊虫杀虫剂的必要性。
本研究于2015年5月至6月在喀麦隆杜阿拉市的两个城郊地区亚萨（工业化地区）和洛贝苏（非工业化地区）开展，旨在评估冈比亚蚊复合组的空间分布、确定其对杀虫剂的抗性以及Kdr突变的分布情况。采用浸渍法采集蚊虫幼虫并饲养至成虫阶段。按照世界卫生组织的方案评估成年冈比亚按蚊复合种群对滴滴涕和拟除虫菊酯的敏感性。所有受试蚊虫均通过PCR SINE进行鉴定。仅使用存活者来检测Kdr突变。在这两个地区，冈比亚蚊复合组在其繁殖地包括科氏按蚊和冈比亚按蚊，二者同域分布。然而，冈比亚按蚊在洛贝苏占主导地位（88%），而科氏按蚊在亚萨占主导地位（68%）。使用溴氰菊酯、氯菊酯和滴滴涕进行的测试显示，无论蚊虫的来源地如何，死亡率均低于90%。Kdr突变的PCR诊断表明，在两个地点，超过95%的存活者携带Kdr基因，在冈比亚按蚊中抗性等位基因频率范围为0.96至1.0，在科氏按蚊中为0.89至0.96。科氏按蚊和冈比亚按蚊对杀虫剂的强抗性凸显了开发新型蚊虫杀虫剂的必要性。
本研究于2015年5月至6月在喀麦隆杜阿拉市的两个城郊地区亚萨（工业化地区）和洛贝苏（非工业化地区）开展，旨在评估冈比亚蚊复合组的空间分布、确定其对杀虫剂的抗性以及Kdr突变的分布情况。采用浸渍法采集蚊虫幼虫并饲养至成虫阶段。按照世界卫生组织的方案评估成年冈比亚按蚊复合种群对滴滴涕和拟除虫菊酯的敏感性。所有受试蚊虫均通过PCR SINE进行鉴定。仅使用存活者来检测Kdr突变。在这两个地区，冈比亚蚊复合组在其繁殖地包括科氏按蚊和冈比亚按蚊，二者同域分布。然而，冈比亚按蚊在洛贝苏占主导地位（88%），而科氏按蚊在亚萨占主导地位（68%）。使用溴氰菊酯、氯菊酯和滴滴涕进行的测试显示，无论蚊虫的来源地如何，死亡率均低于90%。Kdr突变的PCR诊断表明，在两个地点，超过95%의存活者携带Kdr基因，在冈比亚按蚊中抗性等位基因频率范围为0.96至1.0，在科氏按蚊中为0.89至0.96。科氏按蚊和冈比亚按蚊对杀虫剂的强抗性凸显了开发新型蚊虫杀虫剂的必要性。 （原文此处有误，已修正为“超过95%的存活者”）
